Minimum Essential Medium Eagle/Nutrient Mixture F12 Ham (MEM/F12 1:1 Mixture)With 15 mM HEPES buffer
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Catalog number
AT237-20LX2
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Price
Please ask
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Size
22X0L
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Description
Minimum Essential Medium Eagle/Nutrient Mixture F12 Ham (MEM/F12 1:1 Mixture)With 15 mM HEPES buffer used in laboratory chemicals, Manufacture of substances
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Shelf Life
Use before the expiry date
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Storage conditions
store between 2-8°C
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Appearance
NA
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Solubility
NA
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Use
used in laboratory chemicals, Manufacture of substances
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Technical data
Please contact us to request the technical data
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Data sheet
Please contact us to request the technical data
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(no name)
Please contact us to request the data sheet
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Additional description
Buffering solutions are useful to keep the pH range sable when using this reagent of Himedia. There are two major types of growth media those used for cell culture , which use specific cell types derived from plants or animals, and microbiological culture, which are used for growing microorganisms, such as bacteria or yeast. The most common growth media for microorganisms are nutrient broths and agar plates with antibiotics; specialized media are sometimes required for microorganism and cell culture growth.
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Buffer
25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.
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Group
media
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Gene target
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Gene symbol
F12
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Short name
Minimum Essential Medium Eagle/Nutrient Mixture F12 Ham (MEM/F12 1:1 Mixture)With 15 mM
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Technique
Medium, cell culture Media
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Alternative name
Minimum Essential Medium Eagle/Nutrient Mixture F12 Ham (MEM/F12 1:1 Mixture)including 15 millimeter HEPES solution
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Alternative technique
cellculture
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Virus
hhv
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Gene info
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Identity
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Gene
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Long gene name
coagulation factor XII
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Synonyms gene name
- coagulation factor XII (Hageman factor)
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GenBank acession
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Locus
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Discovery year
2001-06-22
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Entrez gene record
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RefSeq identity
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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Concept
Scope note:
Methods to study reactions or processes taking place in an artificial environment outside the living organism.
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Tree numbers
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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