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Description
Minimum Essential Medium Eagle (MEM), With Earle's salts Without L-Glutamine, NEAA, Phenol red and Sodium bicarbonate, is an off-white to creamish white, homogenous powder. It was developed to meet the specific nutritional requirements of certain subtypes of Hela cells and normal mammalian fibroblasts. It can be modified by eliminating calcium to facilitate growth of cells in suspension cultures.
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Shelf life
Use before expiry date given on the product label.
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Storage
Store at 2-8°C away from bright light
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Appearance
Off-white to Creamish white, homogenous powder.
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PH without Sodium Bicarbonate
5.90 -6.50
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PH with Sodium Bicarbonate
7.30 -7.90
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Osmolality without Sodium Bicarbonate
220.00 -260.00
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Osmolality with Sodium Bicarbonate
260.00 -300.00
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Cultural Response
The growth promotion capacity of the medium is assessed qualitatively by analyzing the cells for the morphology and quantitatively by estimating the cell counts and comparing it with a control medium through minimum three subcultures.
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Endotoxin Content
NMT 5EU/ml
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Solubility
Clear solution at 9.3 gms/L
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Uses
Laboratory chemicals, Manufacture of substances
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Personal Protection
Use personnel protective equipment. Wear disposable gloves, dust mask and eye protection. Avoid dust formation.
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Toxicological Information
NA
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Ordering
To order Minimum Essential Medium Eagle (MEM) With Earle's salts Without L-Glutamine, NEAA, Phenol red and Sodium bicarbonate, please use the Cat. Nr.AT159A-5X10X1L and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
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Tips
Our specialists recommend you to follow carefully the pre-registered instructions for Minimum Essential Medium Eagle (MEM) With Earle's salts Without L-Glutamine, NEAA, Phenol red and Sodium bicarbonate
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Additional description
There are two major types of growth media those used for cell culture , which use specific cell types derived from plants or animals, and microbiological culture, which are used for growing microorganisms, such as bacteria or yeast. The most common growth media for microorganisms are nutrient broths and agar plates with antibiotics; specialized media are sometimes required for microorganism and cell culture growth.
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Group
media
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Conjugation
red dye