Lowenstein Jensen Medium Base

Description

Lowenstein Jensen Medium Base is used for the isolation and cultivation of Mycobacterium species
Other Product
Alternative Name

L.J. Medium
Type

Dehydrated Culture Media
Origin

Animal
Classification

Bacterial
Composition g L

L-Asparagine 3.600 Monopotassium phosphate 2.400 Magnesium sulphate 0.240 Magnesium citrate 0.600 Potato starch, soluble 30.000 Malachite green 0.400
Quantity required per litre

31,93 g
Appearance

Greenish blue to peacock blue homogeneous
Colour and Clarity of prepared medium

The mixture of sterile basal medium and whole egg emulsion, when inspissated, coagulates to yield pale bluish green coloured, opaque smooth slants
Cultural response

Cultural characteristics observed in presence of 5-10% Carbon dioxide, with added egg emulsion base, after an incubation at 35-37ºC for 2-4 weeks.
Storage

Store below 30°C in tightly closed container and the prepared medium at 2-8°C
Shelf Life

Use before expiry date on the label.
Technical Data
Data Sheet
Additional description

There are two major types of growth media those used for cell culture , which use specific cell types derived from plants or animals, and microbiological culture, which are used for growing microorganisms, such as bacteria or yeast. The most common growth media for microorganisms are nutrient broths and agar plates with antibiotics; specialized media are sometimes required for microorganism and cell culture growth.
Group

media

Name

Primary Cell Culture
Concept

Scope note: The initial culturing of cells derived directly from fresh TISSUES.
Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data