HiCrome Nickels and Leesment Medium

• Catalog number
  M1712-8X100G
• Price
  Please ask
• Size
  8X100G

• Description
  HiCrome Nickels and Leesment Medium is employed to enumerate citrate-fermenting lactic acid bacteria from milk, milk products and mesophilic starter cultures.
• Other Product
• Alternative Name
  NA
• Type
  Dehydrated Culture Media
• Origin
  Animal
• Classification
  Bacterial
• Composition g L
  Casein enzymic hydrolysate 18.000 Yeast extract 4.500 Gelatine 2.250 Glucose 4.500 Lactose 4.500 Sodium chloride 3.600 Trisodium citrate dihydrate 1.800 Calcium lactate pentahydrate 8.000 Tricalcium dicitrate tetrahydrate 6.650 Carboxymethyl cellulose (CMC) 0.400 Chromogenic substrate (X-gal) 0.200 Agar 15.000 Final pH ( at 25°C) 6.65±0.05
• Quantity required per litre
  66 g
• Appearance
  Cream to light yellow homogeneous free flowing powder
• Colour and Clarity of prepared medium
  White coloured, opaque gel containing white precipitate forms in Petri plates.
• Cultural response
  Cultural characteristics observed after an incubation at 25-30°C for 48-72 hours.
• Storage
  Store dehydrated powder and prepared medium at 2-8°C
• Shelf Life
  Use before expiry date on the label.
• Technical Data
• Data Sheet
• Additional description
  There are two major types of growth media those used for cell culture , which use specific cell types derived from plants or animals, and microbiological culture, which are used for growing microorganisms, such as bacteria or yeast. The most common growth media for microorganisms are nutrient broths and agar plates with antibiotics; specialized media are sometimes required for microorganism and cell culture growth.
• Group
  media

• Gene target
  HiCrome   Nickels   Leesment  
• Short name
  HiCrome Nickels Leesment Medium
• Technique
  Medium, cell culture Media
• Alternative name
  HiCrome Nickels and Leesment Medium
• Alternative technique
  cellculture
• Virus
  hhv

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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