Dulbecco's Modified Eagle Medium /Nutrient Mixture F-12 Ham (DMEM/F12, 1:1 mixture) w/ L-Alanyl-L-Glutamine and Trace elements w/o HEPES buffer and Sodium bicarbonate

  • Catalog number
    AT127G-2X10X1L
  • Price
    Please ask
  • Size
    2X10X1L
  • Description
    Dulbecco's Modified Eagle Medium /Nutrient Mixture F-12 Ham (DMEM/F12, 1:1 mixture), w/ L-Alanyl-L-Glutamine and Trace elements w/o HEPES buffer and Sodium bicarbonate, is a white to light pink, homogenous powder that is suitable for cell culture.
  • Similar products
  • Shelf life
    Use before expiry date given on the product label.
  • Storage
    Store at 2-8°C away from bright light
  • Appearance
    White to light pink, homogenous powder
  • PH without Sodium Bicarbonate
    NA
  • PH with Sodium Bicarbonate
    NA
  • Osmolality without Sodium Bicarbonate
    NA
  • Osmolality with Sodium Bicarbonate
    NA
  • Cultural Response
    NA
  • Endotoxin Content
    NA
  • Solubility
    NA
  • Uses
    Laboratory chemicals, Manufacture of substances
  • Personal Protection
    Use personnel protective equipment. Wear disposable gloves, dust mask and eye protection. Avoid dust formation.
  • Toxicological Information
    NA
  • Technical Data
    Contact us to request a Technical Data
  • Data sheet
  • Ordering
    To order Dulbecco's Modified Eagle Medium /Nutrient Mixture F-12 Ham (DMEM/F12, 1:1 mixture) w/ L-Alanyl-L-Glutamine and Trace elements w/o HEPES buffer and Sodium bicarbonate, please use the Cat. Nr.AT127G-2X10X1L and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
  • Tips
    Our specialists recommend you to follow carefully the pre-registered instructions for Dulbecco's Modified Eagle Medium /Nutrient Mixture F-12 Ham (DMEM/F12, 1:1 mixture) w/ L-Alanyl-L-Glutamine and Trace elements w/o HEPES buffer and Sodium bicarbonate
  • Additional description
    Buffering solutions are useful to keep the pH range sable when using this reagent of Himedia. There are two major types of growth media those used for cell culture , which use specific cell types derived from plants or animals, and microbiological culture, which are used for growing microorganisms, such as bacteria or yeast. The most common growth media for microorganisms are nutrient broths and agar plates with antibiotics; specialized media are sometimes required for microorganism and cell culture growth.
  • Buffer
    25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.
  • Group
    media
  • Gene target
    Dulbecco's   Modified   Eagle   Nutrient   Mixture   F-12   Ham   DMEM/F12   mixture   L-Alanyl-L-Glutamine   Trace   elements   w/o   Sodium   bicarbonate  
  • Gene symbol
    F12, CPT1A, POM121L2, L3MBTL2, CLDN10, RPL32P1, FABP1, COX7A2, NFIA, MAS1L
  • Short name
    Dulbecco's Modified Eagle Medium /Nutrient Mixture F-12 Ham (DMEM/F12, 1:1 mixture) w/ L-Alanyl-L-Glutamine Trace elements w/o Sodium bicarbonate
  • Technique
    Medium, cell culture Media
  • Alternative name
    Dulbecco's Modified Eagle Medium /Nutrient Mixture F-12 Ham (DMEM/F12, 1:1 mixture) w/ L-Alanyl-L-Glutamine and Trace elements w/o HEPES solution and Sodium bicarbonate
  • Alternative technique
    cellculture
  • Virus
    hhv
Gene info
Gene info
Gene info
  • Identity
    HGNC:13973
  • Gene
    POM121L2
  • Long gene name
    POM121 transmembrane nucleoporin like 2
  • Synonyms gene name
    • POM121 membrane glycoprotein-like 2 (rat)
    • POM121 membrane glycoprotein-like 2
    • POM121 transmembrane nucleoporin-like 2
  • Synonyms
  • GenBank acession
  • Locus
    6p22.1
  • Discovery year
    2001-07-20
  • Entrez gene record
    94026
  • RefSeq identity
  • VEGA ID
    OTTHUMG00000014475
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
MeSH Data
  • Name
    Primary Cell Culture
  • Concept
    Scope note: The initial culturing of cells derived directly from fresh TISSUES.
  • Tree numbers
    • E01.370.225.500.223.500
    • E05.200.500.265.500
    • E05.242.223.500
    • E05.481.500.249.500
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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Dulbecco s Modified Eagle Medium Nutrient Mixture F 12 Ham D
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