Dulbecco's Modified Eagle Medium/ Nutrient Mixture F-12 Ham (DMEM/F12, 1:1 Mixture) w/ Glutamine, 15mM HEPES buffer, Trace elements, 0.04mM Calcium chloride
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Catalog numberAT216-20LX2
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PricePlease ask
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Size2X20L
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DescriptionDulbecco's Modified Eagle Medium / Nutrient Mixture F-12 Ham (DMEM/F12, 1:1 mixture) by himedia laboratory was originally formulated for rat neuroblastoma cells and MDCK celles is a Nutrient Mixture F-12 Ham with Lglutamine, 15mM HEPES buffer, trace elements and 0.04mM Calcium chloride.
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Shelf LifeUse before the expiry date
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Storage conditionsstored at 2-8°C.
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AppearanceOff-white to Creamish white, homogenous powder
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SolubilityNA
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Useused in laboratory chemicals, Manufacture of substances
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Additional descriptionBuffering solutions are useful to keep the pH range sable when using this reagent of Himedia. There are two major types of growth media those used for cell culture , which use specific cell types derived from plants or animals, and microbiological culture, which are used for growing microorganisms, such as bacteria or yeast. The most common growth media for microorganisms are nutrient broths and agar plates with antibiotics; specialized media are sometimes required for microorganism and cell culture growth.
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Buffer25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.
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Groupmedia
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Gene target
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Gene symbolF12, HLA-F, SNAR-F, H3C11, IGHV1-69-2, KLRC4, PIRC55, PIRC54, PIRC53
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Short nameDulbecco's Modified Eagle Medium/ Nutrient Mixture F-12 Ham (DMEM/F12, 1:1 Mixture) w/ Glutamine, 15mM , Trace elements, 04mM Calcium chloride
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TechniqueMedium, cell culture Media
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Alternative nameDulbecco's Modified Eagle Medium/ Nutrient Mixture F-12 Ham (DMEM/F12, 1:1 Mixture) w/ Glutamine, 15mM HEPES solution, Trace elements, 0.04mM Calcium chloride
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Alternative techniquecellculture
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Virushhv
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Gene info
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Identity
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Gene
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Long gene namecoagulation factor XII
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Synonyms gene name
- coagulation factor XII (Hageman factor)
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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RefSeq identity
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namemajor histocompatibility complex, class I, F
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GenBank acession
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Locus
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Discovery year1990-09-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- C1-set domain containing
- Histocompatibility complex
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namesmall NF90 (ILF3) associated RNA F
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Synonyms gene name
- small nuclear ILF3/NF90-associated RNA F
- small ILF3/NF90-associated RNA F
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GenBank acession
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Locus
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Discovery year2008-06-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small NF90 (ILF3) associated RNAs
Gene info
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Identity
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Gene
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Long gene nameH3 clustered histone 11
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Synonyms gene
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Synonyms gene name
- H3 histone family, member F
- histone 1, H3i
- histone cluster 1, H3i
- histone cluster 1 H3 family member i
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-08-27
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- H3 histones
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 1-69-2
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Synonyms gene
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Synonyms gene name
- immunoglobulin heavy variable 1-F (provisional)
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namekiller cell lectin like receptor C4
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Synonyms gene name
- killer cell lectin-like receptor subfamily C, member 4
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-09-03
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Killer cell lectin like receptors
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 55
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 54
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 53
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data