Dulbecco’s Modified Eagle Medium (DMEM), Low glucose w/ 1gm Glucose per litre, Sodium bicarbonate w/o L-Glutamine, Sodium pyruvate, HEPES and Phenol red

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  • Catalog number
    AL277-40X500ML
  • Price:

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  • Size
    40X500ML
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  • Description
    Dulbecco’s Modified Eagle Medium (DMEM), Low glucose w/ 1gm Glucose per litre, Sodium bicarbonate w/o L-Glutamine, Sodium pyruvate, HEPES and Phenol red, is a Colorless, clear solution. DMEM is a modification of Basal Medium Eagle (BME) that contains a four-fold higher concentration of amino acids and vitamins, as well as additional supplementary components. DMEM is used for culturing embryonic mouse cells.
  • Shelf life
    Use before expiry date given on the product label.
  • Storage
    Store at 2-8°C away from bright light
  • Appearance
    Colorless, clear solution
  • PH
    NA
  • Osmolality in mOsm Kg H2O
    NA
  • Sterility
    NA
  • Cultural Response
    NA
  • Endotoxin Content
    NA
  • Uses
    Laboratory chemicals, Manufacture of substances
  • Personal Protection
    Use personnel protective equipment. Wear disposable gloves, dust mask and eye protection. Avoid dust formation.
  • Toxicological Information
    NA
  • Technical Data
    Contact us to request a Technical Data
  • Ordering
    To order Dulbecco’s Modified Eagle Medium (DMEM), Low glucose w/ 1gm Glucose per litre, Sodium bicarbonate w/o L-Glutamine, Sodium pyruvate, HEPES and Phenol red, please use the Cat. Nr.AL277-40X500ML and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
  • Tips
    Our specialists recommend you to follow carefully the pre-registered instructions for Dulbecco’s Modified Eagle Medium (DMEM), Low glucose w/ 1gm Glucose per litre, Sodium bicarbonate w/o L-Glutamine, Sodium pyruvate, HEPES and Phenol red
  • Buffer
    25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.
  • Additional description
    The Liter or liter is a metric system unit of volume equal to 1 cubic decimeter (dm3), 1,000 cubic centimeters (cm3) or 1/1,000 cubic meter. A cubic decimeter (or liter) occupies a volume of 10×10×10 centimeters (see figure) and is thus equal to one-thousandth of a cubic meter. Water baths of various volumes are available for lab research. There are two major types of growth media those used for cell culture , which use specific cell types derived from plants or animals, and microbiological culture, which are used for growing microorganisms, such as bacteria or yeast. The most common growth media for microorganisms are nutrient broths and agar plates with antibiotics; specialized media are sometimes required for microorganism and cell culture growth.
  • Group
    media
  • Conjugation
    red dye
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  • Gene target
  • Short name
    Dulbecco’s Modified Eagle Medium (DMEM), glucose w/ 1gm Glucose per , Sodium bicarbonate w/o L-Glutamine, Sodium pyruvate, Phenol red
  • Technique
    Medium, cell culture Media
  • Label
    Red
  • Alternative name
    Dulbecco’s Modified Eagle Medium (DMEM), Low glucose w/ 1gm Glucose per litre, Sodium bicarbonate w/o L-Glutamine, Sodium pyruvate, HEPES and Phenol red
  • Alternative technique
    cellculture
  • Virus
    hhv
MeSH Data
  • Name
  • Concept
    Scope note: Methods to study reactions or processes taking place in an artificial environment outside the living organism.
  • Tree numbers
    • E05.481
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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