Dulbecco's Modified Eagle Medium/ Nutrient Mixture F-12 Ham (DMEM/ F12, 1:1 mixture) w/ L-Glutamine, 2mg/L Riboflavin and 55mg/L Sodium pyruvate and Sodium bicarbonate w/o HEPES buffer, Trace elements and Pyridoxal hydrochloride

  • Catalog number
    AL243A-30X500ML
  • Price
    Please ask
  • Size
    30X500ML
  • Description
    Dulbecco's Modified Eagle Medium/ Nutrient Mixture F-12 Ham (DMEM/ F12, 1:1 mixture) w/ L-Glutamine, 2mg/L Riboflavin and 55mg/L Sodium pyruvate and Sodium bicarbonate w/o HEPES buffer, Trace elements and Pyridoxal hydrochloride, is a red colored, clear solution. It was originally formulated for rat neuroblastoma cells and MDCK cells.
  • Shelf life
    Use before expiry date given on the product label.
  • Storage
    Store at 2-8°C away from bright light
  • Appearance
    Red colored, clear solution
  • PH
    NA
  • Osmolality in mOsm Kg H2O
    NA
  • Sterility
    NA
  • Cultural Response
    NA
  • Endotoxin Content
    NA
  • Uses
    Laboratory chemicals, Manufacture of substances
  • Personal Protection
    Use personnel protective equipment. Wear disposable gloves, dust mask and eye protection. Avoid dust formation.
  • Toxicological Information
    NA
  • Technical Data
    Contact us to request a Technical Data
  • Ordering
    To order Dulbecco's Modified Eagle Medium/ Nutrient Mixture F-12 Ham (DMEM/ F12, 1:1 mixture) w/ L-Glutamine, 2mg/L Riboflavin and 55mg/L Sodium pyruvate and Sodium bicarbonate w/o HEPES buffer, Trace elements and Pyridoxal hydrochloride, please use the Cat. Nr.AL243A-30X500ML and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
  • Tips
    Our specialists recommend you to follow carefully the pre-registered instructions for Dulbecco's Modified Eagle Medium/ Nutrient Mixture F-12 Ham (DMEM/ F12, 1:1 mixture) w/ L-Glutamine, 2mg/L Riboflavin and 55mg/L Sodium pyruvate and Sodium bicarbonate w/o HEPES buffer, Trace elements and Pyridoxal hydrochloride
  • Additional description
    Buffering solutions are useful to keep the pH range sable when using this reagent of Himedia. There are two major types of growth media those used for cell culture , which use specific cell types derived from plants or animals, and microbiological culture, which are used for growing microorganisms, such as bacteria or yeast. The most common growth media for microorganisms are nutrient broths and agar plates with antibiotics; specialized media are sometimes required for microorganism and cell culture growth.
  • Buffer
    25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.
  • Group
    media
  • Gene target
  • Gene symbol
    F12, HLA-F, H3C11, IGHV1-69-2, SNAR-F, KLRC4, PIRC55, PIRC57, PIRC53
  • Short name
    Dulbecco's Modified Eagle Medium/ Nutrient Mixture F-12 Ham (DMEM/ F12, 1:1 mixture) w/ L-Glutamine, 2mg/L Riboflavin 55mg/L Sodium pyruvate Sodium bicarbonate w/o , Trace elements Pyridoxal hydrochloride
  • Technique
    Medium, cell culture Media
  • Alternative name
    Dulbecco's Modified Eagle Medium/ Nutrient Mixture F-12 Ham (DMEM/ F12, 1:1 mixture) w/ L-Glutamine, 2mg/L Riboflavin and 55mg/L Sodium pyruvate and Sodium bicarbonate w/o HEPES solution, Trace elements and Pyridoxal hydrochloride
  • Alternative technique
    cellculture
  • Virus
    hhv
Gene info
Gene info
Gene info
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    small NF90 (ILF3) associated RNA F
  • Synonyms gene name
    • small nuclear ILF3/NF90-associated RNA F
    • small ILF3/NF90-associated RNA F
  • GenBank acession
  • Locus
  • Discovery year
    2008-06-20
  • Entrez gene record
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • Small NF90 (ILF3) associated RNAs
Gene info
  • Identity
  • Gene
  • Long gene name
    killer cell lectin like receptor C4
  • Synonyms gene name
    • killer cell lectin-like receptor subfamily C, member 4
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    1998-09-03
  • Entrez gene record
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • Killer cell lectin like receptors
  • VEGA ID
Gene info
  • Identity
  • Gene
  • Long gene name
    piwi-interacting RNA cluster 55
  • Locus
    12
  • Discovery year
    2009-11-05
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Piwi-interacting RNA clusters
Gene info
  • Identity
  • Gene
  • Long gene name
    piwi-interacting RNA cluster 57
  • Locus
    12
  • Discovery year
    2009-11-05
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Piwi-interacting RNA clusters
Gene info
  • Identity
  • Gene
  • Long gene name
    piwi-interacting RNA cluster 53
  • Locus
    12
  • Discovery year
    2009-11-05
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Piwi-interacting RNA clusters
MeSH Data
  • Name
  • Concept
    Scope note: Methods to study reactions or processes taking place in an artificial environment outside the living organism.
  • Tree numbers
    • E05.481
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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