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Description
Bolton Broth Base is used to enrich selectively Campylobacter species from foods
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Alternative Name
NA
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Type
Dehydrated Culture Media
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Origin
Animal
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Classification
Bacterial
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Composition g L
Enzymatic digest of animal tissues 10.000 Lactalbumin hydrolysate 5.000 Yeast extract 5.000 Sodium chloride 5.000 Sodium metabisulphite 0.500 Sodium carbonate 0.600 Hemin 0.010 alpha-ketoglutaric Acid 1.000 Sodium pyruvate 0.500 Final pH ( at 25°C) 7.4±0.2
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Quantity required per litre
27,6 g
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Appearance
Light yellow to brownish yellow homogeneous free flowing powder
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Colour and Clarity of prepared medium
Basal medium : Brownish yellow coloured clear to slightly opalescent solution. After addition of lysed horse blood: Red to brown coloured opaque solution in tubes
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Cultural response
Cultural characteristics observed with added Bolton Selective Supplement (FD231) after an incubation at 35-37°C for 4-6 hours and then at 41.5°C for 40-48 hours
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Storage
Store below 30°C in tightly closed container and prepared medium at 2-8°C
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Shelf Life
Use before expiry date on the label.
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Properties
º C. The broth is a modified culture medium for culturing isolated or enriched bacteria, yeasts and micro organisms.The buffered pH and low salt content of these media broths will ensure a perfect growth of the pathogens to be detected. The broth or culture medium is supplied in 500gr and 5 x 500gr quntities by Himedia. The broth's MSDS certificate is supplied for appoval of our broth's batch quality. The screening and selective enrichment of these pathogens and bacteriacan also be obtained by other broths and selective culture media supplements.
