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Description
BHI Broth is used to propagate fastidious pathogenic cocci and other organisms associated with blood culture work and allied pathological investigations, also used for the enrichment of Staphylococcus aureus
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Alternative Name
Brain Heart Infusion Broth
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Type
Dehydrated Culture Media
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Origin
Animal
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Classification
Bacterial
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Composition g L
Peptic digest of animal tissue 10.000 Calf brain, infusion (solids) 12.500 Beef heart, infusion (solids) 5.000 Dextrose 2.000 Sodium chloride 5.000 Disodium phosphate 2.500 Final pH ( at 25°C) 7.4±0.2
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Quantity required per litre
37 g
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Appearance
Cream to yellow homogeneous free flowing powder
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Colour and Clarity of prepared medium
Light amber coloured, clear to slightly opalescent solution
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Cultural response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
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Storage
Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C
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Shelf Life
Use before expiry date on the label.
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Properties
º C. The broth is a modified culture medium for culturing isolated or enriched bacteria, yeasts and micro organisms.The buffered pH and low salt content of these media broths will ensure a perfect growth of the pathogens to be detected. The broth or culture medium is supplied in 500gr and 5 x 500gr quntities by Himedia. The broth's MSDS certificate is supplied for appoval of our broth's batch quality. The screening and selective enrichment of these pathogens and bacteriacan also be obtained by other broths and selective culture media supplements.
