Basal Medium Eagle (BME) With Earle's Salts, L-Glutamine and 25mM HEPES buffer Without Sodium bicarbonate
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Catalog numberAT061-10X5L
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Price:
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Size10X5L
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DescriptionBasal Medium Eagle (BME), With Earle's Salts, L-Glutamine and 25mM HEPES buffer Without Sodium bicarbonate, is an off-white to creamish white, homogenous powder. It was initially developed as a result of studies to determine the nutritional requirements of HeLa cells and mouse fibroblast L cells in culture. When properly supplemented supports growth of variety of diploid or primary mammalian cell cultures.
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Shelf lifeUse before expiry date given on the product label.
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StorageStore at 2-8°C away from bright light
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AppearanceOff-white to Creamish white, homogenous powder.
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PH without Sodium Bicarbonate5.00 -5.60
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PH with Sodium Bicarbonate6.70 -7.30
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Osmolality without Sodium Bicarbonate280.00 -320.00
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Osmolality with Sodium Bicarbonate330.00 -370.00
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Cultural ResponseThe growth promotion capacity of the medium is assessed qualitatively by analyzing the cells for the morphology and quantitatively by estimating the cell counts and comparing it with a control medium through minimum three subcultures.
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Endotoxin ContentNMT 5EU/ml
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SolubilityClear solution at 15.2 gms/L.
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UsesLaboratory chemicals, Manufacture of substances
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Personal ProtectionUse personnel protective equipment. Wear disposable gloves, dust mask and eye protection. Avoid dust formation.
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Toxicological InformationNA
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OrderingTo order Basal Medium Eagle (BME) With Earle's Salts, L-Glutamine and 25mM HEPES buffer Without Sodium bicarbonate, please use the Cat. Nr.AT061-10X5L and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
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TipsOur specialists recommend you to follow carefully the pre-registered instructions for Basal Medium Eagle (BME) With Earle's Salts, L-Glutamine and 25mM HEPES buffer Without Sodium bicarbonate
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Additional descriptionBuffering solutions are useful to keep the pH range sable when using this reagent of Himedia. There are two major types of growth media those used for cell culture , which use specific cell types derived from plants or animals, and microbiological culture, which are used for growing microorganisms, such as bacteria or yeast. The most common growth media for microorganisms are nutrient broths and agar plates with antibiotics; specialized media are sometimes required for microorganism and cell culture growth.
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Buffer25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.
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Groupmedia
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Gene target
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Short nameBasal Medium Eagle (BME) With Earle's Salts, L-Glutamine 25mM Without Sodium bicarbonate
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TechniqueMedium, cell culture Media
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Alternative nameBasal Medium Eagle (BME) including Earle's Salts, L-Glutamine and 25mM HEPES solution Without Sodium bicarbonate
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Alternative techniquecellculture
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Virushhv
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MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data