Immunoglobulin G (IgG) (H+L) F(ab')2, Rat -biotin conjugated

Old catalog number

10-251-409061
Storage temperature

store cold
Expiry date

1 y
Category

research antibodies, ELISAs, recombinant proteins, vectors and reagents for laboratory use
Properties

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation

Biotinylated
Group

f(ab')2s
About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Latin name

Rattus norvegicus

Gene target

Immunoglobulin IgGH+LFab
Short name

Immunoglobulin G (IgG) (H+L) F(ab')2, - conjugated
Technique

F(ab')2, IgG, antibody Conjugates, F(ab')2 antibody fragments are the antigen binding variable regions obtained by enzymatic cutting of the parent IgG at neutral ph. IgGs
Host

Rat
Isotype

IgG, F(ab')2 Fragment Affinity-Purified Secondary Antibodies, IgG
Label

Biotin
Species

Rat, Rats
Alternative name

Immunoglobulin G (Immunoglobulin G) (heavy + light chain) F(Antibody')2, Rat -biotinilated coupled
Alternative technique

secondairy, immunoglobulins