E. coli 0157:H7 -FITC conjugated

Old catalog number

18-251-404404
Storage temperature

store cold
Expiry date

1 y
Category

research antibodies, ELISAs, recombinant proteins, vectors and reagents for laboratory use
Properties

This genways Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. genways FITC is highly sensitive to pH extremes.
Conjugation

Anti-FITC Antibody

Gene target

coli 0157:H7
Short name

0157:H7 -FITC conjugated
Technique

FITC, antibody Conjugates, Fluorescein
Host

e. coli, Escherichia coli, Recombination of bioactive proteins and longer peptides in Escherichia Coli is done often with His tagging. genways supplies Rec. E. Coli affinity purified or tag purified antigens in 1 quantities or bulk volumes on request.
Label

FITC
Species

E. coli, E. coli
Alternative name

E. coli 0157:H7 -fluorecein coupled
Alternative technique

escherichia, fluorescine

Name

Cytophotometry
Concept

Scope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data