VetPCR MHV Mouse hepatitis virus RT-PCR
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Catalog number
597-VET-EX003-96R
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Price
Please ask
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Size
1 kit or item
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How to order
The reference at Genprice for this lab product is 597-VET-EX003-96R. It is a frequent ordered reagent.
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When will I be delivered
The VetPCR MHV Mouse hepatitis virus RT-PCR will be delivered to your laboratory the next week after your order if you order it before Friday 15:30.
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Contact with other users
VetPCR MHV Mouse hepatitis virus RT-PCR is frequently used in research laboratories. We can supply you with references if needed.
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Description
Hepatitis a, b, c, d, e recombinant surface viral antigens to genotype 1, 2, 3, 4, 5 are used for antibody production to make diagnostic poly or monoclonal antibodies. Hepatitis is a disease of the liver characterized by the presence of inflammatory cells in the tissue of the organ. Hepatitis may occur without symptoms, but can lead to jaundice (a yellow discoloration of the skin, mucous membranes, and conjunctiva of the eyes), poor appetite, and fatigue. Depending on the cause, hepatitis can manifest either as an acute or as a chronic disease.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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Group
PCR, polymerase chain reaction, RT-PCR mixes
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About
TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
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Properties
Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
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Gene target
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Short name
VetPCR MHV Mouse hepatitis virus RT-PCR
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Technique
RT-PCR, Mouse, PCR, mouses, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment.
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Host
mouse
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Species
Virus, Mouses, Viruses
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Alternative name
VetPCR MHV Mouse hepatitis virus Reverse transcription PCR test kit
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Alternative technique
rtpcrkits, murine, dna-amplification
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Virus
hepatitis
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MeSH Data
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Name
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Concept
Scope note:
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
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Tree numbers
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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