Genomic DNA Isolation Kit (Blood/Cultured Cell/Fungus)
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Catalog number
PDC09-0100
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Price
Please ask
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Size
100 rxns
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Description
Genomic DNA Isolation Kit is used for genomic DNA isolation from the whole blood, frozen blood, buffy coat, cultured animal/bacterial cells and fungus. This unique buffer system ensures genomic DNA with high yield and good quality from samples.
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Storage
Store at room temperature other than RNase A (-20ºC)
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Shelflife
Use before expiry date given on the label
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Sample
Up to 300 µl of the whole blood Up to 200 µl of the frozen blood Up to 200 µl of the buffy coat Cultured animal cells (up to 1 x 107 ) Cultured bacterial cells (up to 1 x 109 ) Fungus cells (up to 5 x 107 )
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Format
Reagent and spin column
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Operation time
Within 60 minutes
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Elution volume
50∼200 μl
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Yield
Up to 50 μg
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Endotoxin value
NA
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Contents
Buffer CR / Buffer CC 35 ml / Buffer CB / Buffer W1 / Buffer W2 (Add ethanol) / Buffer E 10 ml 1 ml Columns CC / Collection Tubes
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PHYSICAL AND CHEMICAL PROPERTIES
Buffer CR: Form: liquid Miscibility with water: immiscible pH：8.0// Buffer CC: Form: liquid Miscibility with water: immiscible pH：8.5 // Buffer CB: Form: liquid Miscibility with water: immiscible pH：5.5 // Buffer W1 Form: liquid Miscibility with water: immiscible pH：7.0 // Buffer W2 Form: liquid Miscibility with water: immiscible pH：7.5 // Buffer EL Form: liquid Miscibility with water: immiscible pH：8.5
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Uses
For laboratory use
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Ordering
To order Genomic DNA Isolation Kit (Blood/Cultured Cell/Fungus), please use the Cat. Nr.PDC09-0100 and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
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Tips
Our specialists recommend you to follow carefully the pre-registered instructions for Genomic DNA Isolation Kit (Blood/Cultured Cell/Fungus)
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Additional description
Blood is slightly basic and contains proteins, hemoglobin, albumins in its serum. GeneDireX (Bio-Helix) supplies this blood related product in 1. For cells, cell lines and tissues in culture till half confluency.
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Gene target
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Gene symbol
PRKDC, HLA-DOA
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Short name
Genomic DNA Isolation Kit (Blood/Cultured /Fungus)
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Technique
blood, dna, blood samples
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Alternative name
Genomic Desoxyribonucleic acid purification reagent (Blood/Cultured cellular/Fungus)
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Alternative technique
sera, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Tissue
blood, cell
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Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
Methods of maintaining or growing biological materials in controlled laboratory conditions. These include the cultures of CELLS; TISSUES; organs; or embryo in vitro. Both animal and plant tissues may be cultured by a variety of methods. Cultures may derive from normal or abnormal tissues, and consist of a single cell type or mixed cell types.
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Tree numbers
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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