2X 1 Step RT-PCR Master Mix

Product type

PCR/RT-PCR Amplification kits
Category

PCR/RT-PCR Amplification kits
Group

PCR, polymerase chain reaction, RT-PCR mixes
About

TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
Properties

Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR. Master mixes of Taq polymerases are supplied with dNTP's and Mg+buffer already premixed. They are used for automation and to exclude variaions in your PCR.

Gene target

Master
Short name

2X 1 Step RT-PCR Master
Technique

RT-PCR, step, PCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment. steps
Alternative name

2X 1 Step Reverse transcription PCR test kit Master Mix
Alternative technique

rtpcrkits, steps, dna-amplification

Name

Nucleic Acid Amplification Techniques
Concept

Scope note: Laboratory techniques that involve the in-vitro synthesis of many copies of DNA or RNA from one original template.
Tree numbers
- E05.393.620
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data