chicken polyclonal anti-FLAG (DYKDDDDK) (HRP) epitope tag antibody

• Catalog number
  AFLAG-HRP
• Price
  Please ask
• Size
  100 ug

• Antigen
  FLAG (DYKDDDDK) (HRP)
• Tech file
• Chicken IgY
  Gallus gallus domesticus and other IgY antibodies have high affinity and are easy to collect from the yolk. Gallus gallus can produce 20 more than in rabbit polyclonal. The antibody will not bind to rheumatoid factor or protein or Fc regions. This gives very much reduced background very much suited for diagnostic applications. Papain will digest IgY’s to divalent Fab antibody fragments. IgY’s are suited for Elisa kit development, Immunohistochemistry and Western Blot.
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  If you buy Antibodies supplied by gallus IgY they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Chickens like all bird species have IgY antibodies that are very stable and found in the yolk. Gallus Gallus domestica is the Latin name of the Chicken. An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv.  These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies. HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
• Conjugation
  Flag, HRP Conjugated
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
• French translation
  anticorps

• Gene target
  DYKDDDDK   epitope   tag  
• Short name
  polyclonal anti- (DYKDDDDK) ( ) epitope tag antibody
• Technique
  Polyclonal, Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope. Tags
• Label
  Flag
• Species
  chicken, Chickens
• Alternative name
  chicken polyclonal Antibody toFLAG (DYKDDDDK) (horseradish peroxidase) epitope detection labelled (antibody to-)
• Alternative technique
  polyclonals, antibodies

MeSH Data

• Name
  Epitope Mapping
• Concept
  Scope note: Methods used for studying the interactions of antibodies with specific regions of protein antigens. Important applications of epitope mapping are found within the area of immunochemistry.
• Tree numbers
  • E05.478.274
  • E05.601.690.300
• Qualifiers
  ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data

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