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Category
Proteins
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Antibody Subtype
Recombinant Proteins
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Area of research
Nutrition & Metabolism
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Reactivity
Human
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Residues
Region of Visfatin protein corresponding to amino acids MPPNTSKVYS YFECREKKTE NSKLRKVKYE ETVFYGLQYI LNKYLKGKVV TKEKIQEAKD VYKEHFQDDV FNEKGWNYIL EKYDGHLPIE IKAVPEGFVI PRGNVLFTVE NTDPECYWLT NWIETILVQS WYPITVATNS REQKKILAKY LLETSGNLDG LEYKLHDFGY RGVSSQETAG IGASAHLVNF KGTDTVAGLA LIKKYYGTKD PVPGYSVPAA EHSTITAWGK DHEKDAFEHI VTQFSSVPVS VVSDSYDIYN ACEKIWGEDL RHLIVSRSTQ APLIIRPDSG NPLDTVLKVL EILGKKFPVT ENSKGYKLLP PYLRVIQGDG VDINTLQEIV EGMKQKMWSI ENIAFGSGGG LLQKLTRDLL NCSFKCSYVV TNGLGINVFK DPVADPNKRS KKGRLSLHRT PAGNFVTLEE GKGDLEEYGQ DLLHTVFKNG KVTKSYSFDE IRKNAQLNIE LEAAHH.
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Type of protein
Recombinant
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Expression System
Escherichia coli
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Grade Purity
Grater than 98% pure
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Form Buffer
Lyophilized from 10 mM citric acid, pH 2.8. Reconstitute with laboratory grade water.
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Storage
Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
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Shipping conditions
Blue Ice
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Tested for
Please refer to the datasheet
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Bioactivity
The ED50 was determined by the dose-dependant proliferation of the RPMI 8226 cells. The expected ED50 for this effect is 15.0-20.0 ng/ml.
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