Vimentin antibody FITC
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Catalog number61R-V104aFT
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PricePlease ask
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Size250 µl
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CategoryPrimary Antibody
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Antibody SubtypeConjugated Monoclonal Antibodies
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Area of researchCell Biology
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Type of ImmunogenVimentin antibody (FITC) was raised in mouse using vimentin purified from bovine lens ..
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Raised inMouse
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SpecificityBovine vimentin
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Cross ReactivityAmphibia, bovine, chicken, dog, human, monkey
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IsotypesIgG2a
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CloneVIM 3B4
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Conjugate TagFITC
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Method of PurificationProtein A affinity chromatography
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Form BufferProtein A purified and Provided as a liquid
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StorageStore at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
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Shipping conditionsBlue Ice
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Tested forELISA; IF; IHC-F; IHC-P; WB
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Usage RecommendationsIHC-F: 1:10, IHC-P: 1:10
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PropertiesIf you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.
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ConjugationAnti-FITC Antibody
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French translationanticorps
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Gene target
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Gene symbolVIM2P, VIM, VMAC
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Short nameVimentin antibody FITC
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TechniqueAntibody, FITC, antibodies against human proteins, antibodies for, Fluorescein
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LabelFITC
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Alternative nameMouse monoclonal Vimentin antibody (FITC)
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Alternative techniqueantibodies, fluorescine
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Gene info
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Identity
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Gene
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Long gene namevimentin 2, pseudogene
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Synonyms gene
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Synonyms gene name
- vimentin pseudogene 1
- vimentin 2, pseudogene (functional)
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Synonyms
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Synonyms name
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Locus
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Discovery year2012-04-23
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namevimentin
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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RefSeq identity
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Classification
- Intermediate filaments Type III
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namevimentin type intermediate filament associated coiled-coil protein
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GenBank acession
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Locus
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Discovery year2009-06-12
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data