VEGF protein His tag

• Catalog number
  80R-1195
• Price
  Please ask
• Size
  50 µg

• Category
  Proteins
• Antibody Subtype
  Conjugated Proteins
• Area of research
  Cytokines & Growth Factors
• Reactivity
  Mouse
• Residues
  205-324 amino acids: MGSSHHHHHH SSGLVPRGSH MAPTTEGEQK SHEVIKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNITMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKCDKPR R
• Conjugate Tag
  His tag
• Type of protein
  Recombinant
• Expression System
  Escherichia coli
• Grade Purity
  Grater than 90% pure
• Concentration
  0.5 mg/ml (lot specific)
• Form Buffer
  Provided as a liquid in 20 mM Tris-HCl buffer, pH 8.0, containing 10% glycerol.
• Storage
  Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
• Shipping conditions
  Blue Ice
• Tested for
  SDS-PAGE
• Bioactivity
  The ED50 for this effect is 0.5-1.5 ng/ml. Measured in a cell proliferation assay using NIH-3T3 mouse embryonic fibroblast. The ED50 for this effect is 0.5-1.5 ng/ml.
• Assay Information
  1. Cell line: NIH-3T3 (Mus mosculus/Mouse embryonic fibroblast ) 2. Maintenance Condition: RPMI 1640 containing 10% FBS 3. Assay medium: serum free RPMI 1640 4. Cell density: 2 x 10,000 cells/well (96 well plate, final volume 100ul) 5. Incubation time: 24 hr (after sample treatment) 6. Concentration range: 1 pg/ml – 1 ug/ml 7. Detection method : BrdU assay
• Additional Information
  VEGF protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
• URL
• More info
• Conjugation
  histidine

• Gene target
  VEGF   protein   His   tag  
• Gene symbol
  VEGFA, VEGFC, VEGFD
• Short name
  VEGF protein His tag
• Technique
  Tags
• Label
  His
• Alternative name
  Purified recombinant Mouse VEGF protein

Gene info

• Identity
  HGNC:12680
• Gene
  VEGFA
• Long gene name
  vascular endothelial growth factor A
• Synonyms gene
  • VEGF
• Synonyms gene name
  • vascular endothelial growth factor
• Synonyms
  • VEGF-A
  • VPF
• GenBank acession
  • AB021221
• Locus
  6p21.1
• Discovery year
  1994-01-10
• Entrez gene record
  7422
• Pubmed identfication
  • 8786112
• RefSeq identity
  • NM_001025366
• Classification
  • VEGF family
• VEGA ID
  OTTHUMG00000014745
• Locus Specific Databases
  • ALSOD, the Amyotrophic Lateral Sclerosis Online Genetic Database

Gene info

• Identity
  HGNC:12682
• Gene
  VEGFC
• Long gene name
  vascular endothelial growth factor C
• Synonyms
  • VRP
  • VEGF-C
• Synonyms name
  • vascular endothelial growth factor-related protein
• GenBank acession
  • BC035212
• Locus
  4q34.3
• Discovery year
  1996-10-26
• Entrez gene record
  7424
• Pubmed identfication
  • 8617204
• RefSeq identity
  • NM_005429
• Classification
  • VEGF family
• VEGA ID
  OTTHUMG00000160734

Gene info

• Identity
  HGNC:3708
• Gene
  VEGFD
• Long gene name
  vascular endothelial growth factor D
• Synonyms gene
  • FIGF
• Synonyms gene name
  • c-fos induced growth factor (vascular endothelial growth factor D)
• Synonyms
  • VEGF-D
• GenBank acession
  • AJ000185
• Locus
  Xp22.2
• Discovery year
  1997-03-27
• Entrez gene record
  2277
• Pubmed identfication
  • 9479493
• RefSeq identity
  • NM_004469
• Classification
  • VEGF family
• VEGA ID
  OTTHUMG00000021175
• Locus Specific Databases
  • Global Variome shared LOVD

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