Toll-like receptor 4 antibody biotin
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Catalog number
61R-1681
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Price
Please ask
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Size
50 µg
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Category
Primary Antibody
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Antibody Subtype
Conjugated Monoclonal Antibodies
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Area of research
Signal Transduction
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Raised in
Rat
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Specificity
Mouse
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Isotypes
IgG2a kappa
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Clone
MTS510
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Conjugate Tag
Biotin
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Concentration
500 ug/ml
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Form Buffer
Provided in aqueous buffer with 0.09% NaN3, may contain carrier protein/stabilizer.
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Storage
Store at 2-8 deg C. Do not freeze. Protect from light.
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Shipping conditions
Blue Ice
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Tested for
FC
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Usage Recommendations
FC: ≤ 0.5 ug/test
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Assay Information
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10⁵ to 10⁸ cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
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Properties
If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Conjugation
Biotinylated
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Description
The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
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French translation
anticorps
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Gene target
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Gene symbol
TLR4
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Short name
Toll-like receptor 4 antibody
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Technique
Antibody, antibodies against human proteins, antibodies for
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Label
Biotin
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Alternative name
Rat monoclonal Toll-like receptor 4 antibody (biotin)
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Alternative technique
antibodies
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Gene info
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Identity
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Gene
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Long gene name
toll like receptor 4
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Synonyms gene name
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Synonyms
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GenBank acession
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Locus
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Discovery year
1998-06-25
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Toll like receptors
- CD molecules
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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