TNF alpha antibody biotin
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Catalog number
61R-1663
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Price
Please ask
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Size
50 µg
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Category
Primary Antibody
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Antibody Subtype
Conjugated Monoclonal Antibodies
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Area of research
Cytokines & Growth Factors
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Raised in
Mouse
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Specificity
Mouse, Rat
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Isotypes
IgG1 kappa
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Clone
MAb11
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Conjugate Tag
Biotin
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Concentration
500 ug/ml
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Form Buffer
Provided in aqueous buffer with 0.09% NaN3, may contain carrier protein/stabilizer.
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Storage
Store at 2-8 deg C. Do not freeze. Protect from light.
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Shipping conditions
Blue Ice
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Tested for
FC
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Usage Recommendations
FC: ≤ 0.5 ug/test
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Assay Information
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10⁵ to 10⁸ cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
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Description
The TNF alpha antibody is a α- or alpha protein sometimes glycoprotein present in blood.
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Properties
If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Conjugation
Biotinylated
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Gene
Tumor necrosis factor (TNFa, tumor necrosis factor alpha, TNFα, cachexin, or cachectin) is a cell signaling protein (cytokine) involved in systemic inflammation and is one of the cytokines that make up the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. TNFb or TNF beta also bin on TNF receptors for Th1 activation.
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French translation
anticorps
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Gene target
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Gene symbol
TNF, TNFRSF1A, TNFRSF1B, LTBR
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Short name
TNF alpha antibody
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Technique
Antibody, antibodies against human proteins, antibodies for
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Label
Biotin
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Alternative name
Mouse monoclonal TNF alpha antibody (biotin)
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Alternative technique
antibodies
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Alternative to gene target
tumor necrosis factor, DIF and TNF-alpha and TNFA and TNFSF2, TNF and IDBG-300259 and ENSG00000232810 and 7124, transcription regulatory region DNA binding, Extracellular, Tnf and IDBG-177358 and ENSMUSG00000024401 and 21926, TNF and IDBG-634161 and ENSBTAG00000025471 and 280943
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Gene info
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Identity
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Gene
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Long gene name
tumor necrosis factor
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Synonyms gene
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Synonyms gene name
- tumor necrosis factor (TNF superfamily, member 2)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
1986-01-01
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Entrez gene record
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Pubmed identfication
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Classification
- Tumor necrosis factor superfamily
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VEGA ID
Gene info
Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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