SIRT1 protein His tag

Category

Proteins
Antibody Subtype

Conjugated Proteins
Area of research

DNA & RNA
Reactivity

Human
Residues

254-495 aa: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMKK IIVLTGAGVS VSCGIPDFRS RDGIYARLAV DFPDLPDPQA MFDIEYFRKDPRPFFKFAKE IYPGQFQPSL CHKFIALSDK EGKLLRNYTQ NIDTLEQVAG IQRIIQCHGS FATASCLICK YKVDCEAVRG DIFNQVVPRC PRCPADEPLA IMKPEIVFFG ENLPEQFHRA MKYDKDEVDL LIVIGSSLKV RPVALIPSSI PHEVPQILIN REPLPHLHFD VELLGDCDVIINELCHRLGG
Conjugate Tag

His tag
Type of protein

Recombinant
Expression System

Escherichia coli
Grade Purity

Grater than 90% pure
Method of Purification

SIRT1 protein was purified by proprietary chromatographic techniques
Concentration

500 ug/ml
Form Buffer

Provided in liquid form in 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.4M Urea
Storage

Can be stored at 4 deg C short term. For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles
Shipping conditions

Blue Ice
Tested for

SDS-PAGE
URL
More info
Conjugation

histidine

Gene target

SIRT1 protein His tag
Gene symbol

SIRT1-AS, SIRT1
Short name

SIRT1 protein His tag
Technique

Tags
Label

His
Alternative name

Purified recombinant SIRT1 protein (His tag)
Alternative to gene target

sirtuin 1, SIR2L1, SIRT1 and IDBG-75847 and ENSG00000096717 and 23411, keratin filament binding, nuclei, Sirt1 and IDBG-159441 and ENSMUSG00000020063 and 93759, SIRT1 and IDBG-629119 and ENSBTAG00000014023 and 613629

Identity

HGNC:14929
Gene

SIRT1
Long gene name

sirtuin 1
Synonyms gene name
- sirtuin (silent mating type information regulation 2, S. cerevisiae, homolog) 1
- sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae)
Synonyms
- SIR2L1
GenBank acession
- AF083106
Locus

10q21.3
Discovery year

2001-03-20
Entrez gene record

23411
Pubmed identfication
- 10381378
RefSeq identity
- NM_012238
Classification
- eNoSC complex
- Sirtuins
VEGA ID

OTTHUMG00000018340