Rat Pan T cell antibody biotin
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Catalog number
61R-T135aBT
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Price
Please ask
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Size
200 µg
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Category
Primary Antibody
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Antibody Subtype
Conjugated Monoclonal Antibodies
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Area of research
Immunology
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Type of Immunogen
Rat pan T cell antibody (biotin) was raised in mouse using rat lymphocytes ..
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Raised in
Mouse
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Specificity
Rat
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Cross Reactivity
Should be determined by the end user
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Isotypes
IgG2a
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Clone
Ki-T1R
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Conjugate Tag
Biotin
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Form Buffer
Lyophilized from PBS, pH 7.2, with 10mg/ml BSA and 0.01% thimerosal. Reconstitute with 0.5 ml of distilled water.
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Storage
Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
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Shipping conditions
Blue Ice
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Tested for
FC; IHC-F
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Usage Recommendations
IHC-F: 0.25 ug/ml (1:800)
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Properties
If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Conjugation
Biotinylated
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Description
For cells, cell lines and tissues in culture till half confluency.
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About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin name
Rattus norvegicus
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French translation
anticorps
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Gene target
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Short name
Pan T antibody
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Technique
Antibody, antibodies against human proteins, antibodies for
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Host
Rat
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Label
Biotin
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Species
Rat, Rats
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Alternative name
Mouse monoclonal Rat Pan T cell antibody (biotin)
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Alternative technique
antibodies
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Tissue
cell
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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