Rabbit Borrelia burgdorferi antibody (FITC)
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Catalog number
60-LR20
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Price
Please ask
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Size
1 ml
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Applications
User optimized
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Product Type
Primary Antibodies
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Product Subtype
Conjugated Polyclonal Antibodies
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Research Area
Infectious Disease
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Immunogen
Borrelia burgdorferi antibody (FITC) was raised in rabbit using a whole cell preparation from Borrelia burgdorferi as the immunogen.
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Specificity
Borrelia burgdorferi
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Cross Reactivity
To be determined by end-user
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Clone
NA
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Concentration
Typically 4-5 mg/ml
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Form Buffer
PBS, pH 7.2, with 0.1% BSA, and 0.1% NaN3.
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Storage
Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
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Shipping Info
Blue Ice
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Properties
If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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About
Rabbits are used for polyclonal antibody production by fitzgerald. Rabbit antibodies are very stable and can be stored for several days at room temperature. fitzgerald adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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French translation
anticorps
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Gene target
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Short name
Rabbit Borrelia burgdorferi antibody (FITC)
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Technique
Antibody, Rabbit, FITC, antibodies against human proteins, antibodies for, Fluorescein
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Host
Rabbit, Rabbits
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Isotype
NA
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Label
FITC
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Alternative name
Rabbit polyclonal Borrelia burgdorferi antibody (FITC) conjugated
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Alternative technique
antibodies, rabbit-anti, fluorescine
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Disease
borrelia
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MeSH Data
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Name
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Concept
Scope note:
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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