-
Category
Secondary Antibody
-
Antibody Subtype
Conjugated Secondary Antibodies
-
Area of research
Immunology
-
Type of Immunogen
Rabbit anti-rat IgM (HRP) was raised in rabbit using rat IgM mu heavy chain ..
-
Raised in
Rabbit
-
Specificity
Rat IgM
-
Cross Reactivity
Should be determined by the end user
-
Conjugate Tag
HRP
-
Method of Purification
Rabbit anti Rat IgM (HRP) was purified by delipidation, selective precipitation and tandem molecular sieve chromatography followed by dialysis.
-
Form Buffer
Lyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0.01% gentamicin sulfate. DO NOT add NaN3. I mMunoglobulin and protease free.
-
Storage
Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
-
Shipping conditions
Blue Ice
-
Tested for
ELISA; IHC; WB
-
Usage Recommendations
ELISA: 1:60,000, IHC: 1:500-1:2,500, WB: 1:1,000-1:5,000
-
-
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
-
Properties
HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot. Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen
-
Conjugation
HRP Conjugated
-
About
Rabbits are used for polyclonal antibody production by fitzgerald. Rabbit antibodies are very stable and can be stored for several days at room temperature. fitzgerald adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
-
Latin name
Oryctolagus cuniculus, Rattus norvegicus
