PPIH protein

  • Catalog number
    30R-1197
  • Price
    Please ask
  • Size
    100 µg
  • Category
    Proteins
  • Antibody Subtype
    Recombinant Proteins
  • Area of research
    Cell Cycle & Cell Death
  • Reactivity
    Human
  • Residues
    1-177 amino acids: MAVANSSPVN PVVFFDVSIG GQEVGRMKIE LFADVVPKTA ENFRQFCTGE FRKDGVPIGY KGSTFHRVIK DFMIQGGDFV NGDGTGVASI YRGPFADENF KLRHSAPGLL SMANSGPSTN GCQFFITCSK CDWLDGKHVV FGKIIDGLLV MRKIENVPTG PNNKPKLPVV ISQCGEM
  • Type of protein
    Recombinant
  • Expression System
    Escherichia coli
  • Grade Purity
    Grater than 95% pure
  • Concentration
    1 mg/ml (lot specific)
  • Form Buffer
    Provided as a liquid in PBS, pH 7.4, containing 10% glycerol.
  • Storage
    Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
  • Shipping conditions
    Blue Ice
  • Tested for
    SDS-PAGE
  • Bioactivity
    Specific activity is > 40 nmoles/min/ug, and is defined as the amount of enzyme that cleaves 1uM of suc-AAFP-pNA per minute at 1 DEG C in Tris-HCl pH 8.0 using chymotrypsin.
  • Assay Information
    1. Prepare assay buffer in a suitable container and pre-chill on ice before use: The final concentrations are 35 mM Tris, pH 8.0, 1uM suc-AAFP-pNA). 2. Add recombinant Cyclophilin H protein with various concentrations (1ug, 2ug, 5ug) in assay buffer. 3. Mix by inversion and equilibrate to 1 DEG C and monitor the A410nm until the value is constant using a spectrophotometer. 4. Add pre-chilled chymotrypsin to 12.5 uM and mix immediately. 5. Record the increase in A410 nm for 20 minutes
  • Additional Information
    PPIH protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
  • URL
  • Gene target
    PPIH   protein  
  • Gene symbol
    PPIH
  • Short name
    PPIH protein
  • Alternative name
    Purified recombinant Human PPIH protein
  • Alternative to gene target
    peptidylprolyl isomerase H (cyclophilin H), PPIH and IDBG-97224 and ENSG00000171960 and 10465, ribonucleoprotein complex binding, nuclei, Ppih and IDBG-182988 and ENSMUSG00000060288 and 433064,66101, PPIH and IDBG-640563 and ENSBTAG00000004590 and 513428
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