PPIH protein
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Catalog number30R-1197
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PricePlease ask
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Size100 µg
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CategoryProteins
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Antibody SubtypeRecombinant Proteins
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Area of researchCell Cycle & Cell Death
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ReactivityHuman
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Residues1-177 amino acids: MAVANSSPVN PVVFFDVSIG GQEVGRMKIE LFADVVPKTA ENFRQFCTGE FRKDGVPIGY KGSTFHRVIK DFMIQGGDFV NGDGTGVASI YRGPFADENF KLRHSAPGLL SMANSGPSTN GCQFFITCSK CDWLDGKHVV FGKIIDGLLV MRKIENVPTG PNNKPKLPVV ISQCGEM
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Type of proteinRecombinant
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Expression SystemEscherichia coli
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Grade PurityGrater than 95% pure
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Concentration1 mg/ml (lot specific)
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Form BufferProvided as a liquid in PBS, pH 7.4, containing 10% glycerol.
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StorageStore at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
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Shipping conditionsBlue Ice
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Tested forSDS-PAGE
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BioactivitySpecific activity is > 40 nmoles/min/ug, and is defined as the amount of enzyme that cleaves 1uM of suc-AAFP-pNA per minute at 1 DEG C in Tris-HCl pH 8.0 using chymotrypsin.
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Assay Information1. Prepare assay buffer in a suitable container and pre-chill on ice before use: The final concentrations are 35 mM Tris, pH 8.0, 1uM suc-AAFP-pNA). 2. Add recombinant Cyclophilin H protein with various concentrations (1ug, 2ug, 5ug) in assay buffer. 3. Mix by inversion and equilibrate to 1 DEG C and monitor the A410nm until the value is constant using a spectrophotometer. 4. Add pre-chilled chymotrypsin to 12.5 uM and mix immediately. 5. Record the increase in A410 nm for 20 minutes
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Additional InformationPPIH protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
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Gene target
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Gene symbolPPIH
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Short namePPIH protein
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Alternative namePurified recombinant Human PPIH protein
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Alternative to gene targetpeptidylprolyl isomerase H (cyclophilin H), PPIH and IDBG-97224 and ENSG00000171960 and 10465, ribonucleoprotein complex binding, nuclei, Ppih and IDBG-182988 and ENSMUSG00000060288 and 433064,66101, PPIH and IDBG-640563 and ENSBTAG00000004590 and 513428
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Gene info
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Identity
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Gene
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Long gene namepeptidylprolyl isomerase H
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Synonyms gene name
- peptidyl prolyl isomerase H (cyclophilin H)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2001-03-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- U4/U6 small nuclear ribonucleoprotein
- Cyclophilin peptidylprolyl isomerases
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VEGA ID