PPIE protein His tag

• Catalog number
  80R-1080
• Price
  Please ask
• Size
  100 µg

• Category
  Proteins
• Antibody Subtype
  Conjugated Proteins
• Area of research
  Proteases, Inhibitors, & Enzymes
• Reactivity
  Human
• Residues
  1-301 amino acids: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMATT KRVLYVGGLA EEVDDKVLHA AFIPFGDITD IQIPLDYETE KHRGFAFVEF ELAEDAAAAI DNMNESELFG RTIRVNLAKP MRIKEGSSRP VWSDDDWLKK FSGKTLEENK EEEGSEPPKA ETQEGEPIAK KARSNPQVYM DIKIGNKPAG RIQMLLRSDV VPMTAENFRC LCTHEKGFGF KGSSFHRIIP QFMCQGGDFT NHNGTGGKSI YGKKFDDENF ILKHTGPGLL SMANSGPNTN GSQFFLTCDK TDWLDGKHVV FGEVTEGLDV LRQIEAQGSK DGKPKQKVII ADCGEYV
• Conjugate Tag
  His tag
• Type of protein
  Recombinant
• Expression System
  Escherichia coli
• Grade Purity
  Grater than 95% pure
• Concentration
  1 mg/ml (lot specific)
• Form Buffer
  Provided as a liquid in 20 mM Tris, pH 8.0.
• Storage
  Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
• Shipping conditions
  Blue Ice
• Tested for
  SDS-PAGE
• Bioactivity
  Specific activity is > 210 nmoles/min/ug, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 1 DEG C in Tris-Hcl pH 8.0 using chymotrypsin.
• Assay Information
  1. Prepare assay buffer in a suitable container and pre-chill on ice before use: The final concentrations are 35 mM Tris, pH 8.0, 1uM suc-AAFP-pNA). 2. Add recombinant Cyclophilin E protein with various concentrations (1ug, 2ug, 5ug) in assay buffer. 3. Mix by inversion and equilibrate to 1C and monitor the A410nm until the value is constant using a spectrophotometer. 4. Add pre-chilled chymotrypsin to 12.5 uM and mix immediately. 5. Record the increase in A410 nm for 20 minutes
• Additional Information
  PPIE protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
• URL
• More info
• Conjugation
  histidine

• Gene target
  PPIE   protein   His   tag  
• Gene symbol
  PPIE
• Short name
  PPIE protein His tag
• Technique
  Tags
• Label
  His
• Alternative name
  Purified recombinant Human PPIE protein
• Alternative to gene target
  peptidylprolyl isomerase E (cyclophilin E), CYP-33 and CYP33, PPIE and IDBG-96746 and ENSG00000084072 and 10450, poly(A) RNA binding, nuclei, Ppie and IDBG-184920 and ENSMUSG00000028651 and 56031, BT.21742 and IDBG-640866 and ENSBTAG00000047314 and 100296550,787681

Gene info

• Identity
  HGNC:9258
• Gene
  PPIE
• Long gene name
  peptidylprolyl isomerase E
• Synonyms
  • CyP-33
  • MGC3736
  • MGC111222
• Synonyms name
  • peptidyl-prolyl cis-trans isomerase E
  • cyclophilin 33
  • cyclophilin E
  • PPIase E
  • rotamase E
  • peptidylprolyl isomerase E, isoform 1
• GenBank acession
  • AF042385
• Locus
  1p34.2
• Discovery year
  1999-07-07
• Entrez gene record
  10450
• Pubmed identfication
  • 9747881
• RefSeq identity
  • NM_006112
• Classification
  • Cyclophilin peptidylprolyl isomerases
  • Spliceosomal C complex
  • Spliceosomal B complex
  • Spliceosomal Bact complex
  • Spliceosomal P complex
  • RNA binding motif containing
• VEGA ID
  OTTHUMG00000009248
• Locus Specific Databases
  • LRG_1053

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