PPID protein His tag

• Catalog number
  80R-1048
• Price
  Please ask
• Size
  100 µg

• Category
  Proteins
• Antibody Subtype
  Conjugated Proteins
• Area of research
  Cell Cycle & Cell Death
• Reactivity
  Human
• Residues
  1-370 amino acids: MGSSHHHHHH SSGLVPRGSH MSHPSPQAKP SNPSNPRVFF DVDIGGERVG RIVLELFADI VPKTAENFRA LCTGEKGIGH TTGKPLHFKG CPFHRIIKKF MIQGGDFSNQ NGTGGESIYG EKFEDENFHY KHDREGLLSM ANAGRNTNGS QFFITTVPTP HLDGKHVVFG QVIKGIGVAR ILENVEVKGE KPAKLCVIAE CGELKEGDDG GIFPKDGSGD SHPDFPEDAD IDLKDVDKIL LITEDLKNIG NTFFKSQNWE MAIKKYAEVL RYVDSSKAVI ETADRAKLQP IALSCVLNIG ACKLKMSNWQ GAIDSCLEAL ELDPSNTKAL YRRAQGWQGL KEYDQALADL KKAQGIAPED KAIQAELLKV KQKIKAQKDK EKAVYAKMFA
• Conjugate Tag
  His tag
• Type of protein
  Recombinant
• Expression System
  Escherichia coli
• Grade Purity
  Grater than 95% pure
• Concentration
  1 mg/ml (lot specific)
• Form Buffer
  Provided as a liquid in PBS, pH 7.4, containing 10% glycerol.
• Storage
  Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
• Shipping conditions
  Blue Ice
• Tested for
  SDS-PAGE
• Bioactivity
  Specific activity is > 250 nM/min/ug, and is defined as the amount of enzyme that cleaves 1uM of suc-AAFP-pNA per minute at 1 DEG C in Tris-Hcl pH 8.0 using chymotrypsin.
• Assay Information
  1. Prepare assay buffer in a suitable container and pre-chill on ice before use: The final concentrations are 35 mM Tris, pH 8.0, 1uM suc-AAFP-pNA). 2. Add recombinant Cyclophilin D protein with various concentrations (1ug, 2ug, 5ug) in assay buffer. 3. Mix by inversion and equilibrate to 1 DEG C and monitor the A410nm until the value is constant using a spectrophotometer. 4. Add pre-chilled chymotrypsin to 12.5 uM and mix immediately. 5. Record the increase in A410 nm for 20 minutes
• Additional Information
  PPID protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
• URL
• More info
• Conjugation
  histidine

• Gene target
  PPID   protein   His   tag  
• Gene symbol
  PPID
• Short name
  PPID protein His tag
• Technique
  Tags
• Label
  His
• Alternative name
  Purified recombinant Human PPID protein
• Alternative to gene target
  peptidylprolyl isomerase D, CYP-40 and CYPD, PPID and IDBG-43002 and ENSG00000171497 and 5481, Hsp90 protein binding, nuclei, Ppid and IDBG-153114 and ENSMUSG00000027804 and 67738, PPID and IDBG-630512 and ENSBTAG00000016680 and 281420

Gene info

• Identity
  HGNC:9257
• Gene
  PPID
• Long gene name
  peptidylprolyl isomerase D
• Synonyms gene name
  • peptidylprolyl isomerase D (cyclophilin D)
• Synonyms
  • CYP-40
  • CypD
• Synonyms name
  • cyclophilin 40
• Locus
  4q32.1
• Discovery year
  1995-08-23
• Entrez gene record
  5481
• Pubmed identfication
  • 8509368
• RefSeq identity
  • NM_005038
• Classification
  • Cyclophilin peptidylprolyl isomerases
  • Tetratricopeptide repeat domain containing
• VEGA ID
  OTTHUMG00000161927

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