PKM2 protein His tag

• Catalog number
  80R-1223
• Price
  Please ask
• Size
  100 µg

• Category
  Proteins
• Antibody Subtype
  Conjugated Proteins
• Area of research
  Cancer
• Reactivity
  Human
• Residues
  1-531 amino acids: MGSSHHHHHH SSGLVPRGSH MSKPHSEAGT AFIQTQQLHA AMADTFLEHM CRLDIDSPPI TARNTGIICT IGPASRSVET LKEMIKSGMN VARLNFSHGT HEYHAETIKN VRTATESFAS DPILYRPVAV ALDTKGPEIR TGLIKGSGTA EVELKKGATL KITLDNAYME KCDENILWLD YKNICKVVEV GSKIYVDDGL ISLQVKQKGA DFLVTEVENG GSLGSKKGVN LPGAAVDLPA VSEKDIQDLK FGVEQDVDMV FASFIRKASD VHEVRKVLGE KGKNIKIISK IENHEGVRRF DEILEASDGI MVARGDLGIE IPAEKVFLAQ KMMIGRCNRA GKPVICATQM LESMIKKPRP TRAEGSDVAN AVLDGADCIM LSGETAKGDY PLEAVRMQHL IAREAEAAIY HLQLFEELRR LAPITSDPTE ATAVGAVEAS FKCCSGAIIV LTKSGRSAHQ VARYRPRAPI IAVTRNPQTA RQAHLYRGIF PVLCKDPVQE AWAEDVDLRV NFAMNVGKAR GFFKKGDVVI VLTGWRPGSG FTNTMRVVPV P
• Conjugate Tag
  His tag
• Type of protein
  Recombinant
• Expression System
  Escherichia coli
• Grade Purity
  Grater than 95% pure
• Concentration
  1 mg/ml (lot specific)
• Form Buffer
  Provided as a liquid in 20 mM Tris-HCl buffer, pH 8.0, containing 10% glycerol.
• Storage
  Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
• Shipping conditions
  Blue Ice
• Tested for
  SDS-PAGE
• Assay Information
  1. Prepare a 150ul reaction mixture into a suitable container. Reaction mixture: 72mM Imidazole ( pH 7.5), 7.6mM ADP, 15mM MgCl2, 72mM KCl, 0.2mM Beta-NADH. 2. Incubate for 10 min at 37C. 3. Add 50ul of 20 units/ml LDH, 50ul of 5mM PEP, and incubate for 10 min at 37C. 4. Add 50ul of recombinant PKM2 protein (in 0.1% BSA / PBS) with various concentrations (0.03ug -1ug). 5. Read the decrease in A340nm in kinetic mode for 10 minutes.
• Additional Information
  PKM2 protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
• URL
• More info
• Conjugation
  histidine

• Gene target
  PKM2   protein   His   tag  
• Gene symbol
  PKM
• Short name
  PKM2 protein His tag
• Technique
  Tags
• Label
  His
• Alternative name
  Purified recombinant Human PKM2 protein
• Alternative to gene target
  pyruvate kinase, muscle, CTHBP and HEL-S-30 and OIP3 and PK3 and PKM2 and TCB and THBP1, PKM2 and IDBG-20117 and ENSG00000067225 and 5315, poly(A) RNA binding, nuclei, Pkm and IDBG-173276 and ENSMUSG00000032294 and 18746, PKM2 and IDBG-645572 and ENSBTAG00000001601 and 512571

Gene info

• Identity
  HGNC:9021
• Gene
  PKM
• Long gene name
  pyruvate kinase M1/2
• Synonyms gene
  • PKM2
• Synonyms gene name
  • pyruvate kinase, muscle
• Synonyms
  • THBP1
  • OIP3
  • PK3
• GenBank acession
  • M23725
• Locus
  15q23
• Discovery year
  2001-06-22
• Entrez gene record
  5315
• Pubmed identfication
  • 2040271
• VEGA ID
  OTTHUMG00000172709

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