Phosphothreonine antibody biotin
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Catalog number
60R-PR002bt
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Price
Please ask
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Size
50 µg
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Category
Primary Antibody
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Antibody Subtype
Conjugated Polyclonal Antibodies
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Area of research
Protein Modification & Stress Response
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Type of Immunogen
Phosphothreonine antibody (biotin) was raised in rabbit using phosphothreonine-KLH and phosvitin mixture ..
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Raised in
Rabbit
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Specificity
Human
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Cross Reactivity
Should be determined by the end user
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Conjugate Tag
Biotin
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Concentration
Batch dependent - please inquire should you have specific requirements
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Form Buffer
PBS, with 0.05% NaN3.
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Storage
Aliquot and store at -20 deg C. Avoid repeated Freeze/Thaw cycles
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Shipping conditions
Blue Ice
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Tested for
ELISA; WB
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Usage Recommendations
ELISA: 1:1,000, WB: 1:1,000
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Properties
If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Conjugation
Biotinylated
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French translation
anticorps
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Gene target
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Short name
Phosphothreonine antibody
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Technique
Antibody, antibodies against human proteins, antibodies for
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Label
Biotin
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Alternative name
Rabbit polyclonal Phosphothreonine antibody (biotin) conjugated
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Alternative technique
antibodies
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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