PGP 9.5 antibody

Category

Primary Antibody
Antibody Subtype

Polyclonal Antibodies
Area of research

Neuroscience
Type of Immunogen

PGP 9.5 antibodies were raised in guinea pig using the peptide GASSEDTLLKDAAKVCR, corresponding to residues 175-191 of soluble cytoplasmic protein human PGP 9.5 ..
Raised in

Guinea Pig
Cross Reactivity

Human, mouse, rat
Form Buffer

Whole Serum with 0.05% NaN3.
Storage

Aliquot and store at -20 deg C. Avoid repeated Freeze/Thaw cycles
Shipping conditions

Blue Ice
Tested for

IHC; WB
Usage Recommendations

WB: 1:1000, IHC: 1:5000
Assay Information

PGP 9.5 Blocking Peptide, catalog no. 33R-10434, is also available for use as a blocking control in assays to test for specificity of this PGP 9.5 antibody. Antibody should be pre-incubated with peptide prior to incubation with tissue/sample. We suggest preincubation for 1 hour at room temperature. A 10^-6 or 10^-5 molar (M) peptide concentration is suggested to block antigen/antibody binding. However, optimal working concentration should be determined by the investigator using a range of peptide dilutions to eliminate or reduce immunoreactivity.
URL
More info
Properties

If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translation

anticorps

Name

Blotting, Western
Concept

Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data