NFkB p50 Blocking Peptide
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Catalog number
33R-10470
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Price
Please ask
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Size
50 µg
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Category
Proteins
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Antibody Subtype
Blocking Peptides
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Area of research
Signal Transduction
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Type of protein
Synthetic
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Form Buffer
Provided in phosphate buffered saline (PBS), pH 7.2, containing 0.1% BSA and 0.02% thimerosal.
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Storage
Store at -20 deg C for short term storage. Aliquot and store at -70 deg c for long term storage, avoid repeated freeze/thaw cycles.
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Shipping conditions
Blue Ice
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Tested for
WB
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Assay Information
The peptide is used for blocking the binding of NFkB p50 antibody (catalog no. 20R-1520). In optimal conditions, it blocks the binding completely in Western blot analysis by incubating the peptide with equal volume of antibody for 30 minutes at 37 deg C.
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Test
You can block the antibody by the specific target amino acid sequence of peptide.
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Properties
blocking peptide
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Gene
NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells) is a protein complex that controls transcription of DNA, cytokine production and cell survival. NF-κB is found in almost all animal cell types and is involved in cellular responses to stimuli such as stress, cytokines, free radicals, ultraviolet irradiation, oxidized LDL, and bacterial or viral antigens. NF-κB plays a key role in regulating the immune response to infection (κ light chains are critical components of immunoglobulins). Incorrect regulation of NF-κB has been linked to cancer, inflammatory and autoimmune diseases, septic shock, viral infection, and improper immune development. NF-κB has also been implicated in processes of synaptic plasticity and memory
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Description
Peptides short amino acid chains or epitopes or blocking antagonists. The shortest peptides are dipeptides, consisting of 2 amino acids joined by a single peptide bond, followed by tripeptides, tetra peptides, ... till polypeptides that are long, continuous, and unbranched synthetic peptide chains. These biological oligomers and polymers can be Solid-phase peptide synthesis (SPPS), or in continue produced for custom peptide synthesis projects. The High-efficiency solid phase peptide synthesis (HE-SPPS) is give very low production costs.
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Gene target
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Gene symbol
NFKB1
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Short name
NFkB p50 Blocking Peptide
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Technique
blocking peptide, Blocking, peptide, blocking peptides, fitzgerald made this blocking amino acid sequence or peptide to block the gene target in a volume of 1. For western blot it is often requested to block your antibody and to see the band of the analyzed protein disappear. This is called a negative control by blocking the WB antibody. peptides
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Alternative name
A synthetic peptide for use as a blocking control in assays to test for specificity of NFkB p50 antibody, catalog no. 20R-1520
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Alternative technique
control, peptides
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Gene info
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