NDRG1 protein His tag
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Catalog number80R-1193
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PricePlease ask
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Size100 µg
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CategoryProteins
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Antibody SubtypeConjugated Proteins
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Area of researchCancer
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ReactivityHuman
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Residues1-394 amino acids: MSREMQDVDL AEVKPLVEKG ETITGLLQEF DVQEQDIETL HGSVHVTLCG TPKGNRPVIL TYHDIGMNHK TCYNPLFNYE DMQEITQHFA VCHVDAPGQQ DGAASFPAGY MYPSMDQLAE MLPGVLQQFG LKSIIGMGTG AGAYILTRFA LNNPEMVEGL VLINVNPCAE GWMDWAASKI SGWTQALPDM VVSHLFGKEE MQSNVEVVHT YRQHIVNDMN PGNLHLFINA YNSRRDLEIE RPMPGTHTVT LQCPALLVVG DSSPAVDAVV ECNSKLDPTK TTLLKMADCG GLPQISQPAK LAEAFKYFVQ GMGYMPSASM TRLMRSRTAS GSSVTSLDGT RSRSHTSEGT RSRSHTSEGT RSRSHTSEGA HLDITPNSGA AGNSAGPKSM EVSCLEHHHH HH
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Conjugate TagHis tag
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Type of proteinRecombinant
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Expression SystemEscherichia coli
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Grade PurityGrater than 95% pure
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Concentration1 mg/ml (lot specific)
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Form BufferProvided as a liquid in 20 mM Tris-HCl buffer, pH 8.0, containing 0.1 mM PMSF, and 10% glycerol.
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StorageStore at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
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Shipping conditionsBlue Ice
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Tested forSDS-PAGE
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BioactivityThe ED50 for this effect is 0.5 - 1.5 ng/ml. Measured in a cell proliferation assay using MCF7 cell.
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Assay Information1. Cell line: MCF7 (Human breast adenocarcinoma cell) 2. Maintenance Condition: RPMI 1640 containing 10% FBS 3. Assay medium: serum free RPMI 1640 4. Cell density: 2 x 10,000 cells/well (96 well plate, final volume 100ul) 5. Incubation time : 40 hrs (after sample treatment) 6. Concentration range: 0.19ng/ml - 50ng/ml 7. Detection method : BrdU assay
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Additional InformationNDRG1 protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
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Conjugationhistidine
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Gene target
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Gene symbolNDRG1
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Short nameNDRG1 protein His tag
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TechniqueTags
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LabelHis
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Alternative namePurified recombinant Human NDRG1 protein
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Alternative to gene targetN-myc downstream regulated 1, CAP43 and CMT4D and DRG-1 and DRG1 and GC4 and HMSNL and NDR1 and NMSL and PROXY1 and RIT42 and RTP and TARG1 and TDD5, NDRG1 and IDBG-36420 and ENSG00000104419 and 10397, cadherin binding, nuclei, Ndrg1 and IDBG-146853 and ENSMUSG00000005125 and 17988, NDRG1 and IDBG-644056 and ENSBTAG00000000711 and 504499
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Gene info
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Identity
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Gene
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Long gene nameN-myc downstream regulated 1
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Synonyms gene
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-12-23
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Entrez gene record
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Pubmed identfication
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Classification
- NDRG family
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VEGA ID
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Locus Specific Databases