Mouse CD45 antibody (biotin)

Applications

FC, IP
Product Type

Primary Antibodies
Product Subtype

Conjugated Monoclonal Antibodies
Research Area

Proteases, Inhibitors, & Enzymes
Immunogen

CD45 antibody (biotin) was raised in mouse using chicken CD45 as the immunogen.
Specificity

Chicken
Cross Reactivity

To be determined by end-user
Clone

LT40
Concentration

0.5 mg/ml
Form Buffer

Supplied as a liquid in PBS, with NaN3 added as preservative.
Storage

Store at 4 deg C.
Shipping Info

Blue Ice
Properties

If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation

Biotinylated
Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin name

Mus musculus
French translation

anticorps

Gene target

CD45
Gene symbol

PTPRCAP, PTPRC
Short name

Mouse CD45 antibody ( )
Technique

Antibody, Mouse, antibodies against human proteins, antibodies for, mouses
Host

Mouse
Isotype

IgM
Label

Biotin
Species

Mouse, Mouses
Alternative name

Mouse monoclonal CD45 antibody (biotin)
Alternative technique

antibodies, murine

Identity

HGNC:9666
Gene

PTPRC
Long gene name

protein tyrosine phosphatase receptor type C
Synonyms gene
- CD45
Synonyms
- LCA
- T200
- GP180
GenBank acession
- Y00062
Locus

1q31.3-q32.1
Discovery year

1986-01-01
Entrez gene record

5788
Pubmed identfication
- 2169617
RefSeq identity
- NM_002838
Classification
- Protein tyrosine phosphatases receptor type
- Fibronectin type III domain containing
- CD molecules
VEGA ID

OTTHUMG00000035702
Locus Specific Databases
- PTPRCbase: Mutation registry for CD45 deficiency
- LRG_95

Name

CD4 Lymphocyte Count
Concept

Scope note: The number of CD4-POSITIVE T-LYMPHOCYTES per unit volume of BLOOD. Determination requires the use of a fluorescence-activated flow cytometer.
Tree numbers
- E01.370.225.500.195.107.595.500.150
- E01.370.225.625.107.595.500.150
- E05.200.500.195.107.595.500.150
- E05.200.625.107.595.500.150
- E05.242.195.107.595.500.150
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data