Luciferase antibody biotin

Category

Primary Antibody
Antibody Subtype

Conjugated Polyclonal Antibodies
Area of research

Proteases, Inhibitors, & Enzymes
Type of Immunogen

Luciferase antibodies were raised in Rabbit using luciferase [Photinus pyralis (Firefly)] .
Raised in

Rabbit
Cross Reactivity

Firefly
Isotypes

IgG
Conjugate Tag

Biotin
Method of Purification

Luciferase antibody was purified by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography
Concentration

10.0 mg/ml
Form Buffer

Lyophilized in 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 0.01% (w/v) NaN3 and 10 mg/mL Bovine Serum Albumin (BSA)
Storage

Can be stored at 4 deg C short term. For long term storage, aliquot and store at -20 deg C
Shipping conditions

Blue Ice
Tested for

ELISA; WB
Usage Recommendations

ELISA: 1:80,000 - 1:300,000; Western Blot: 1:8,000 - 1:30,000
URL
More info
Properties

If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation

Biotinylated
French translation

anticorps

Name

Blotting, Western
Concept

Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
Qualifiers

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