Involucrin antibody biotin

Category

Primary Antibody
Antibody Subtype

Conjugated Monoclonal Antibodies
Area of research

Cancer
Type of Immunogen

Involucrin antibody (biotin) was raised in mouse using human keratinocytes’ involucrin ..
Raised in

Mouse
Cross Reactivity

Dog, gorilla, human, monkey, owl, pig, rat
Isotypes

IgG1
Clone

SY5
Conjugate Tag

Biotin
Method of Purification

Involucrin antibody (biotin) was purified by Protein G chromatography.
Concentration

0.2 mg/ml
Form Buffer

Antibody purified from ascites fluid by protein G chromotography and Provided in 10 mM PBS, pH 7.4, with 0.2% BSA and 0.09% NaN3.
Storage

Store at 4 deg C.
Shipping conditions

Blue Ice
Tested for

ELISA; IF; IHC-F; IHC-P; IP; WB
Usage Recommendations

IHC-F: 1:100-1:200, IHC-P: 1:200 IP: 2 ug/mg protein lysate, WB: 1-2 ug/ml
URL
More info
Properties

If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation

Biotinylated
French translation

anticorps

Name

Blotting, Western
Concept

Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data