IL4 protein His tag
-
Catalog number80R-1111
-
PricePlease ask
-
Size50 µg
-
-
CategoryProteins
-
Antibody SubtypeConjugated Proteins
-
Area of researchCytokines & Growth Factors
-
ReactivityHuman
-
Residues25-153 amino acids: MGSSHHHHHH SSGLVPRGSH MHKCDITLQE IIKTLNSLTE QKTLCTELTV TDIFAASKNT TEKETFCRAA TVLRQFYSHH EKDTRCLGAT AQQFHRHKQL IRFLKRLDRN LWGLAGLNSC PVKEANQSTL ENFLERLKTI MREKYSKCSS
-
Conjugate TagHis tag
-
Type of proteinRecombinant
-
Expression SystemEscherichia coli
-
Grade PurityGrater than 95% pure
-
Concentration0.5 mg/ml (lot specific)
-
Form BufferProvided as a liquid in 20 mM Tris-HCl buffer, pH 8.0, containing 10% glycerol.
-
StorageStore at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
-
Shipping conditionsBlue Ice
-
Tested forSDS-PAGE
-
BioactivityThe ED50 for this effect is
-
Assay InformationCell line: TF1 (human erythroleukemic cell) Maintenance Condition: 10% FBS RPMI 1640 with GM-CSF Assay medium - 10% FBS RPMI 1640 without Gm-CSF Cell density: 2 x 10,000 cells/well (96 well plate, final volume 100ul) Starvation: 24hr, Assay medium Incubation time: 24 hr (after sample treatment) Concentration range: 0.078 ng/ml - 400 ng/ml Detection method: MTT assay
-
Additional InformationIL4 protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
-
Conjugationhistidine
-
GeneInterleukin 4 (IL4) is a cytokine that induces differentiation of naive helper T cells (Th0 cells) to Th2 cells. It is used for dendritic and T cell therapy. Upon activation by IL-4, Th2 cells subsequently produce additional IL-4 in a positive feedback loop. The cell that initially produces IL-4, thus inducing Th0 differentiation, has not been identified, but recent studies suggest that basophils may be the effector cell. It is closely related and has functions similar to Interleukin 13. Recombinant, GMP rec. E. coli interleukin-4 for cell culture supplied by GENTAUR. Free samples on request.
-
Gene target
-
Gene symbolIL4
-
Short nameIL4 protein His tag
-
TechniqueTags
-
LabelHis
-
Alternative namePurified recombinant Human IL4 protein
-
Alternative to gene targetinterleukin 4, BCGF-1 and BCGF1 and BSF-1 and BSF1 and IL-4, IL4 and IDBG-42701 and ENSG00000113520 and 3565, growth factor activity, Extracellular, Il4 and IDBG-172024 and ENSMUSG00000000869 and 16189, IL4 and IDBG-644671 and ENSBTAG00000015957 and 280824
-
Gene info
-
Identity
-
Gene
-
Long gene nameinterleukin 4
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year1988-08-10
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Interleukins
-
VEGA ID