IL1b antibody biotin

Category

Primary Antibody
Antibody Subtype

Conjugated Polyclonal Antibodies
Area of research

Cytokines & Growth Factors
Type of Immunogen

IL1b antibody (biotin) was raised in goat using E. Coli derived recombinant human IL1 beta/IL1F2 ..
Raised in

Goat
Specificity

Humen IL1b
Cross Reactivity

Should be determined by the end user
Isotypes

IgG
Conjugate Tag

Biotin
Form Buffer

Lyophilized from sterile filtered PBS solution with BSA. Reconstitute to a concentration of 50 ug/ml with 1 ml of sterile 20 mM Trizma Base, 150 mM NaCl, pH 7.3 containing 0.1% BSA.
Storage

Store at -20 deg C until reconstitution. Following reconstitution product may be stored at 4 deg C in the short term. For long term storage aliquot and freeze at -20 deg C. Avoid repeated freeze/thaw cycles.
Shipping conditions

Blue Ice
Tested for

ELISA; IHC; WB
Usage Recommendations

ELISA: 2-8 ug/ml, IHC: 515 ug/ml, WB: 0.1 ug/ml
URL
More info
Properties

If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation

Biotinylated
French translation

anticorps

Gene target

IL1b
Gene symbol

IL1B
Short name

IL1b antibody
Technique

Antibody, antibodies against human proteins, antibodies for
Label

Biotin
Alternative name

Goat polyclonal IL1b antibody (biotin) conjugated
Alternative technique

antibodies
Alternative to gene target

interleukin 1, beta, IL-1 and IL1-BETA and IL1F2, IL1B and IDBG-66519 and ENSG00000125538 and 3553, protein domain specific binding, Extracellular, Il1b and IDBG-205936 and ENSMUSG00000027398 and 16176, IL1B and IDBG-634613 and ENSBTAG00000001321 and 281251

Name

Blotting, Western
Concept

Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data