IGF1 protein

Category

Proteins
Antibody Subtype

Recombinant Proteins
Area of research

Cytokines & Growth Factors
Reactivity

Human
Residues

1-115 amino acids: MGPETLCGAE LVDALQFVCG DRGFYFNKPT GYGSSSRRAP QTGIVDECCF RSCDLRRLEM YCAPLKPAKS A
Type of protein

Recombinant
Expression System

Escherichia coli
Grade Purity

Grater than 95% pure
Concentration

1 mg/ml (lot specific)
Form Buffer

Provided as a liquid in PBS, pH 7.4.
Storage

Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Shipping conditions

Blue Ice
Tested for

SDS-PAGE
Bioactivity

The ED50 for this effect is < 3.6 ng/ml. Measured in a cell proliferation assay using MCF7 cell.
Assay Information

Cell line: MCF7 (human) Maintenance Condition: RPMI 1640 containing 10% FBS Assay Medium: serum free RPMI 1640 Cell Density: 2 x 10,000 cells/well (96 well plate, final volume 100ul) Serum Free Starvation: 24hr with RPMI1640 Incubation Time: 40 hr (after sample treatment) Concentration Range: 0.39ng/ml - 25ng/ml Detection Method: BrdU assay
Additional Information

IGF1 protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
URL
More info

Gene target

IGF1 protein
Gene symbol

IGF1
Short name

IGF1 protein
Alternative name

Purified recombinant Human IGF1 protein
Alternative to gene target

insulin-like growth factor 1 (somatomedin C), IGF-I and IGF1A and IGFI, IGF1 and IDBG-53609 and ENSG00000017427 and 3479, growth factor activity, Extracellular, Igf1 and IDBG-182955 and ENSMUSG00000020053 and 16000, IGF1 and IDBG-637164 and ENSBTAG00000011082 and 281239