HIV1 tat protein FITC
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Catalog number65-017003F
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PricePlease ask
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Size1 mg
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CategoryProteins
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Antibody SubtypeConjugated Proteins
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Area of researchInfectious Disease
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ReactivityViral
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Conjugate TagFITC
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Type of proteinRecombinant
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Expression SystemBaculovirus
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Grade PurityGrater than 90% pure
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Concentration500 ug/ml
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Form BufferProvided liquid in PBS.
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StorageShips on dry ice. Upon receipt store at -70°C
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Shipping conditionsDry Ice
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Tested forELISA; WB
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Usage RecommendationsELISA: 1:500-1:1,000, WB: 1:500-1:1,000
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PropertiesThis fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.
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ConjugationAnti-FITC Antibody
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Gene target
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Short nameHIV1 tat protein FITC
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TechniqueFITC, Fluorescein
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LabelFITC
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Alternative namePurified recombinant HIV tat protein (FITC)
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Alternative techniquefluorescine
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Virushiv1
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MeSH Data
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Name
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ConceptScope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data