HIV1, HxB2, HxB3 rev antibody FITC

• Catalog number
  61-006103
• Price
  Please ask
• Size
  1 mg

• Category
  Primary Antibody
• Antibody Subtype
  Conjugated Monoclonal Antibodies
• Area of research
  Infectious Disease
• Type of Immunogen
  HIV1, HxB2, HxB3 Rev antibodies were raised in mouse using purified, full length rev (HIV-1 HxB2, HxB3) produced in E.coli Expression system ..
• Raised in
  Mouse
• Specificity
  HIV1
• Isotypes
  IgG2b
• Clone
  9G2G4D6E8
• Conjugate Tag
  FITC
• Concentration
  1.0 mg/ml
• Form Buffer
  Provided liquid as a purified IgG in PBS buffer, with no preservatives added.
• Storage
  Store lyophilized product at 2-8 deg C short term, following reconstitution, aliquot and store at -70 deg C or lower. Avoid repeated freeze/thaw cycles
• Shipping conditions
  Dry Ice
• Tested for
  ELISA; WB
• Usage Recommendations
  ELISA: 1:500-1:1000, WB: 1:500-1:1000
• Assay Information
  Binds to recombinant rev (HIV-1 HxB2, HxB3) in ELISA and Western Blot.  The antibody is epitope mapped to rev (70-84)
• URL
• More info
• Properties
  If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody
• French translation
  anticorps

• Gene target
  HIV1   HxB2   HxB3   rev  
• Short name
  HIV1, HxB2, HxB3 rev antibody FITC
• Technique
  Antibody, FITC, antibodies against human proteins, antibodies for, Fluorescein
• Label
  FITC
• Alternative name
  Mouse monoclonal HIV1 REV antibody (HxB2/HxB3) (FITC)
• Alternative technique
  antibodies, fluorescine
• Virus
  hiv1

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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