HIV1 gp41 MN protein FITC
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Catalog number
65-040003
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Price
Please ask
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Size
50 µg
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Category
Proteins
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Antibody Subtype
Conjugated Proteins
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Area of research
Infectious Disease
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Reactivity
Viral
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Residues
Amino acids 52-258.
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Conjugate Tag
FITC
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Type of protein
Recombinant
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Expression System
Escherichia coli
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Grade Purity
Grater than 95% pure
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Concentration
50 ug/vial
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Form Buffer
Provided lyophilized in PBS buffer. Reconstitute with laboratory grade water.
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Storage
Store lyophilized product at 2-8 deg C short term, following reconstitution, aliquot and store at -70 deg C or lower. Avoid repeated freeze/thaw cycles
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Shipping conditions
Blue Ice
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Tested for
ELISA; WB
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Usage Recommendations
ELISA: 1:500-1:1000, WB: 1:500-1:1000
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Assay Information
The antigen is recognized by anti-gp41 MAB and HIV-1 serum in ELISA and Western Blot, also recognised by Chassie 8 monoclonals in Western Blot.
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Properties
This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
MeSH Data
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Name
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Concept
Scope note:
A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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