HIV1 gp41 MN protein FITC

  • Catalog number
    65-040003
  • Price
    Please ask
  • Size
    50 µg
  • Category
    Proteins
  • Antibody Subtype
    Conjugated Proteins
  • Area of research
    Infectious Disease
  • Reactivity
    Viral
  • Residues
    Amino acids 52-258.
  • Conjugate Tag
    FITC
  • Type of protein
    Recombinant
  • Expression System
    Escherichia coli
  • Grade Purity
    Grater than 95% pure
  • Concentration
    50 ug/vial
  • Form Buffer
    Provided lyophilized in PBS buffer. Reconstitute with laboratory grade water.
  • Storage
    Store lyophilized product at 2-8 deg C short term, following reconstitution, aliquot and store at -70 deg C or lower. Avoid repeated freeze/thaw cycles
  • Shipping conditions
    Blue Ice
  • Tested for
    ELISA; WB
  • Usage Recommendations
    ELISA: 1:500-1:1000, WB: 1:500-1:1000
  • Assay Information
    The antigen is recognized by anti-gp41 MAB and HIV-1 serum in ELISA and Western Blot, also recognised by Chassie 8 monoclonals in Western Blot.
  • URL
  • Properties
    This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.
  • Conjugation
    Anti-FITC Antibody
  • Gene target
    HIV1   gp41   protein  
  • Short name
    HIV1 gp41 MN protein FITC
  • Technique
    FITC, Fluorescein
  • Label
    FITC
  • Alternative name
    Purified recombinant HIV1 gp41 MN (FITC)
  • Alternative technique
    fluorescine
  • Virus
    hiv1
MeSH Data
  • Name
  • Concept
    Scope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
  • Tree numbers
    • E01.370.225.500.386
    • E05.196.712.516.600.240
    • E05.200.500.386
    • E05.242.386
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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