HIV1 Eli Nef antibody FITC

• Catalog number
  61-002103F
• Price
  Please ask
• Size
  50 µg

• Category
  Primary Antibody
• Antibody Subtype
  Conjugated Monoclonal Antibodies
• Area of research
  Infectious Disease
• Type of Immunogen
  HIV1 Eli Nef antibodies were raised in mouse using HIV1 Eli Nef ..
• Raised in
  Mouse
• Specificity
  HIV1 nef
• Isotypes
  IgG1
• Clone
  3F2
• Conjugate Tag
  FITC
• Concentration
  0.55 mg/ml
• Form Buffer
  Provided liquid in PBS buffer. The FITC is attached to the free amine group.
• Storage
  Store frozen product at -70 deg C or lower. Avoid repeated freeze/thaw cycles
• Shipping conditions
  Blue Ice
• Tested for
  ELISA; WB
• Usage Recommendations
  ELISA: 1:500-1:1000, WB: 1:500-1:1000
• Assay Information
  Reacts with purified, whole virus nef (HIV-1 ELI) in ELISA and Western Blot. The antibody is not epitope mapped.
• URL
• More info
• Properties
  If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody
• French translation
  anticorps

• Gene target
  HIV1   Eli   Nef  
• Short name
  HIV1 Eli Nef antibody FITC
• Technique
  Antibody, FITC, antibodies against human proteins, antibodies for, Fluorescein
• Label
  FITC
• Alternative name
  Mouse monoclonal HIV1 Nef antibody (FITC)
• Alternative technique
  antibodies, fluorescine
• Virus
  hiv1

MeSH Data

• Name
  Cytophotometry
• Concept
  Scope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
• Tree numbers
  • E01.370.225.500.386
  • E05.196.712.516.600.240
  • E05.200.500.386
  • E05.242.386
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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