Hexokinase 2 protein His tag
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Catalog number80R-1023
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Price:
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Size100 µg
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CategoryProteins
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Antibody SubtypeConjugated Proteins
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Area of researchSignal Transduction
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ReactivityHuman
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ResiduesMGSSHHHHHH SSGLVPRGSH MIASHLLAYF FTELNHDQVQ KVDQYLYHMR LSDETLLEIS KRFRKEMEKG LGATTHPTAA VKMLPTFVRS TPDGTEHGEF LALDLGGTNF RVLWVKVTDN GLQKVEMENQ IYAIPEDIMR GSGTQLFDHI AECLANFMDK LQIKDKKLPL GFTFSFPCHQ TKLDESFLVS WTKGFKSSGV EGRDVVALIR KAIQRRGDFD IDIVAVVNDT VGTMMTCGYD DHNCEIGLIV GTGSNACYME EMRHIDMVEG DEGRMCINME WGAFGDDGSL NDIRTEFDQE IDMGSLNPGK QLFEKMISGM YMGELVRLIL VKMAKEELLF GGKLSPELLN TGRFETKDIS DIEGEKDGIR KAREVLMRLG LDPTQEDCVA THRICQIVST RSASLCAATL AAVLQRIKEN KGEERLRSTI GVDGSVYKKH PHFAKRLHKT VRRLVPGCDV RFLRSEDGSG KGAAMVTAVA YRLADQHRAR QKTLEHLQLS HDQLLEVKRR MKVEMERGLS KETHASAPVK MLPTYVCATP DGTEKGDFLA LDLGGTNFRV LLVRVRNGKW GGVEMHNKIY AIPQEVMHGT GDELFDHIVQ CIADFLEYMG MKGVSLPLGF TFSFPCQQNS LDESILLKWT KGFKASGCEG EDVVTLLKEA IHRREEFDLD VVAVVNDTVG TMMTCGFEDP HCEVGLIVGT GSNACYMEEM RNVELVEGEE GRMCVNMEWG AFGDNGCLDD FRTEFDVAVD ELSLNPGKQR FEKMISGMYL GEIVRNILID FTKRGLLFRG RISERLKTRG IFETKFLSQI ESDCLALLQV RAILQHLGLE STCDDSIIVK EVCTVVARRA AQLCGAGMAA VVDRIRENRG LDALKVTVGV DGTLYKLHPH FAKVMHETVK DLAPKCDVSF LQSEDGSGKG AALITAVACR IREAGQR
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Conjugate TagHis tag
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Type of proteinRecombinant
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Expression SystemEscherichia coli
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Grade PurityGrater than 95% pure
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Concentration1 mg/ml (lot specific)
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Form BufferProvided as a liquid in 20 mM Tris-HCl, pH 8.0, containing 10% glycerol.
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StorageStore at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
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Shipping conditionsBlue Ice
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Tested forSDS-PAGE
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BioactivitySpecific activity is 3-4 units/ml obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP. In the coupled mode, one unit will produce 1.0 uM of NADPH per minute as glucose is phosphorylated by ATP at pH 7.4 at 30DEG C.
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Assay Information1. Prepare a 2.57ml reaction mixture in a suitable container: The final concentrations are 39mM triethanolamine, 216 mM D-glucose, 0.74mM ATP, 7.8 mM MgCl2, 1.1 mM beta-NADP, 2.5 units G6PD. 2. Equilibrate to 25 DEG C and monitor at A340nm until the value is constant using a spectrophotometer. 3. Add 5ug of recombinant hexokinase-2 into reaction mixture and mix immediately. 4. Record the increase at A340nm for 5 minutes.
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Additional InformationHexokinase type 2 protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
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Conjugationhistidine
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Gene target
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Short nameHexokinase 2 protein His tag
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TechniqueTags
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LabelHis
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Alternative namePurified recombinant Human Hexokinase 2 protein
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