GSTM2 protein His tag

• Catalog number
  80R-1490
• Price
  Please ask
• Size
  100 µg

• Category
  Proteins
• Antibody Subtype
  Conjugated Proteins
• Area of research
  Proteases, Inhibitors, & Enzymes
• Reactivity
  Human
• Residues
  1-218 amino acids: MGSSHHHHHH SSGLVPRGSH MPMTLGYWNI RGLAHSIRLL LEYTDSSYEE KKYTMGDAPD YDRSQWLNEK FKLGLDFPNL PYLIDGTHKI TQSNAILRYI ARKHNLCGES EKEQIREDIL ENQFMDSRMQ LAKLCYDPDF EKLKPEYLQA LPEMLKLYSQ FLGKQPWFLG DKITFVDFIA YDVLERNQVF EPSCLDAFPN LKDFISRFEG LEKISAYMKS SRFLPRPVFT KMAVWGNK
• Conjugate Tag
  His tag
• Type of protein
  Recombinant
• Expression System
  Escherichia coli
• Grade Purity
  Grater than 95% pure
• Concentration
  0.5 mg/ml (lot specific)
• Form Buffer
  Provided as a liquid in 20 mM Tris-HCl buffer, pH 8.0, containing 10% glycerol, 0.1M NaCl and 1 mM DTT.
• Storage
  Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
• Shipping conditions
  Blue Ice
• Tested for
  SDS-PAGE
• Bioactivity
  Specific activity is < 25 units/mg, and is defined as the amount of enzyme that conjugate 1.0 uM of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25 DEG C.
• Assay Information
  1. Prepare a 1 ml reaction mix into a suitable container: The final concentrations are 97 mM potassium phosphate, 0.97 mM EDTA, 2.5 mM glutathione, reduced, 1.0 mM 1-chloro-2,4-dinitrobenzene (CDNB), 3.2% (v/v) ethanol. 2. Equilibrate to 25 DEG C and monitor at A340nm until the value is constant using a spectrophotometer. 3. Add 50ul of recombinant GSTM2 protein with various concentrations (1ug, 2ug, 5ug) in 950ul reaction buffer. 4. Mix by inversion and record the increase at A340nm for 5 minutes.
• Additional Information
  GSTM2 protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
• URL
• More info
• Conjugation
  histidine

• Gene target
  GSTM2   protein   His   tag  
• Gene symbol
  GSTM2
• Short name
  GSTM2 protein His tag
• Technique
  Tags
• Label
  His
• Alternative name
  Purified recombinant Human GSTM2 protein

Gene info

• Identity
  HGNC:4634
• Gene
  GSTM2
• Long gene name
  glutathione S-transferase mu 2
• Synonyms gene name
  • glutathione S-transferase M2 (muscle)
  • glutathione S-transferase mu 2 (muscle)
• Synonyms
  • GST4
• GenBank acession
  • M63509
• Locus
  1p13.3
• Discovery year
  1991-12-06
• Entrez gene record
  2946
• Pubmed identfication
  • 2034681
  • 2345169
• RefSeq identity
  • NM_000848
• Classification
  • Soluble glutathione S-transferases
• VEGA ID
  OTTHUMG00000011638

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