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Category
Secondary Antibody
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Antibody Subtype
Conjugated Secondary Antibodies
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Area of research
Immunology
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Type of Immunogen
Goat anti-mouse IgG (H+L) (Fab'2) (HRP) was raised in goat using murine IgG whole molecule ..
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Raised in
Goat
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Specificity
Murine IgG (H + L)
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Cross Reactivity
Should be determined by the end user
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Conjugate Tag
HRP
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Method of Purification
Goat anti Mouse IgG (H + L) (Fab'2) (HRP) was purified by affinity chromatography.
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Form Buffer
Lyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0.01% gentamycin sulfate. DO NOT add NaN3. I mMunoglobulin and protease free.
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Storage
Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
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Shipping conditions
Blue Ice
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Tested for
ELISA; IHC; WB
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Usage Recommendations
ELISA: 1:60,000, IHC: 1:500-1:2,500, WB: 1:1,000-1:10,000, IHC: 1:500 - 1:2,500, User Optimized
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. This is against a protein of mus musculus directed antibody.
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Properties
Human Fab Of Normal IgG Chromatographically purified Fab fragment of human IgG. Purified human Fab may be used as a reference antigen, calibrator, coating protein and blocking agent, HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
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Latin name
Capra aegagrus hircus, Mus musculus
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Conjugation
HRP Conjugated
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Gene target
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Gene symbol
FUT1, L3MBTL2
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Short name
Goat anti Mouse IgG H + L Fab'2
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Technique
anti mouse, Mouse, anti, Goat, Fab, IgG, antibody to, anti mouse antibodies, fabs, goats, IgGs, mouses
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Host
Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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Isotype
fab'2, IgG, Fab fragment, IgG
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Label
HRP
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Species
Mouse, Goats, Mouses
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Alternative name
Goat anti Mouse IgG (H + L) secondary antibody (Fab'2) (HRP)
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Alternative technique
murine, antibodies, caprine, seconairy, immunoglobulins
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