Goat anti Llama IgG H + L biotin
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Catalog number
43R-1282
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Price
Please ask
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Size
1 mg
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Category
Secondary Antibody
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Antibody Subtype
Conjugated Secondary Antibodies
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Area of research
Immunology
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Type of Immunogen
Purified Llama IgG (H&L)
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Raised in
Goat
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Specificity
This antibody reacts with heavy chains on llama IgG and light chains on all llama immunoglobulins.
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Cross Reactivity
No reactivity is observed to non-immunoglobulin llama serum proteins.
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Conjugate Tag
Biotin
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Grade Purity
Grater than 95% pure
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Method of Purification
Affinity purified using solid phase Llama IgG (H&L)
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Source
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
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Concentration
1.0 mg/ml (lot specific)
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Form Buffer
Clear, colorless liquid, containing 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, 1% (w/v) BSA and 0.05% (w/v) NaN3. Protease/IgG free, pH 7.2.
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Storage
Store at 2-8 deg C.
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Shipping conditions
Blue Ice
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Tested for
Please refer to the datasheet
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Usage Recommendations
1:2000-1:20,000 ELISA/WB, 1:500-1:5000 IHC
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Conjugation
Biotinylated
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Latin name
Capra aegagrus hircus
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Anti llama or alpaca camelloid antibodies to detect the antibodies of the domesticated South American camelid, widely used as production animal with phage display of recombinant llama antibodies.
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Gene target
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Gene symbol
CALD1, FUT1, L3MBTL2
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Short name
Goat anti IgG H + L
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Technique
anti, Goat, IgG, antibody to, goats, IgGs
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Host
Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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Isotype
IgG, IgG
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Label
Biotin
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Species
Llama, Goats, Llamas
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Alternative name
Goat anti Llama IgG (H + L) secondary antibody (biotin)
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Alternative technique
antibodies, caprine, immunoglobulins
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Gene info
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Identity
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Gene
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Long gene name
caldesmon 1
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Synonyms
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GenBank acession
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Locus
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Discovery year
1992-07-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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