Goat anti Human kappa chain HRP
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Catalog number
43R-1260
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Price
Please ask
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Size
500 µg
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Category
Secondary Antibody
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Antibody Subtype
Conjugated Secondary Antibodies
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Area of research
Immunology
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Type of Immunogen
Raised using purified Human Kappa Chain
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Raised in
Goat
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Specificity
This antibody reacts with kappa light chains on human immunoglobulins.
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Cross Reactivity
No reactivity is observed to non-immunoglobulin human serum proteins heavy chains on human immunoglobulins lambda light chains on human immunoglobulins.
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Conjugate Tag
HRP
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Grade Purity
Grater than 95% pure
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Method of Purification
Affinity purified using solid phase Human Kappa Chain
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Source
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
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Concentration
1.0 mg/ml (lot specific)
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Form Buffer
Lyophilized powder containing 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, 1% (w/v) BSA and 0.1% (v/v) Kathon CG. Protease/IgG free, pH 7.2. Rehydrate with 0.55 ml of deionized water. Prepare fresh working dilution daily.
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Storage
Store lyophilized material at 2-8 deg C.
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Shipping conditions
Blue Ice
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Tested for
Please refer to the datasheet
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Usage Recommendations
1:200-1:5000 ELISA/WB, 1:500-1:5000 IHC
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
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Latin name
Capra aegagrus hircus
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Properties
HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Conjugation
HRP Conjugated
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Gene target
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Gene symbol
PPM1K
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Short name
Goat anti kappa chain
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Technique
anti human, anti, Goat, antibody to, anti human antibodies, goats
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Host
Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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Label
HRP
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Species
Human, Goats, Humans
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Alternative name
Goat anti Human kappa chain secondary antibody (HRP)
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Alternative technique
antibodies, caprine
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Gene info
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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