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Category
Proteins
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Antibody Subtype
Conjugated Proteins
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Area of research
Signal Transduction
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Reactivity
E.coli
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Residues
1-367 amino acids of E.coli GLDA protein: MGSSHHHHHH SSGLVPRGSH MGSMDRIIQS PGKYIQGADV INRLGEYLKP LAERWLVVGD KFVLGFAQST VEKSFKDAGL VVEIAPFGGE CSQNEIDRLR GIAETAQCGA ILGIGGGKTL DTAKALAHFM GVPVAIAPTI ASTDAPCSAL SVIYTDEGEF DRYLLLPNNP NMVIVDTKIV AGAPARLLAA GIGDALATWF EARACSRSGA TTMAGGKCTQ AALALAELCY NTLLEEGEKA MLAAEQHVVT PALERVIEAN TYLSGVGFES GGLAAAHAVH NGLTAIPDAH HYYHGEKVAF GTLTQLVLEN APVEEIETVA ALSHAVGLPI TLAQLDIKED VPAKMRIVAE AACAEGETIH NMPGGATPDQ VYAALLVADQ YGQRFLQEWE
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Conjugate Tag
His tag
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Type of protein
Recombinant
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Expression System
Escherichia coli
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Grade Purity
Grater than 95% pure
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Concentration
1 mg/ml
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Form Buffer
Provided as liquid in PBS (pH 7.4) containing 10% glycerol
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Storage
Store at 4 deg C for short term. For long term storage, aliquot and store at -20 deg C. Avoid repeated freeze thaw cycles.
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Shipping conditions
Blue Ice
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Tested for
SDS-PAGE; Control
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Bioactivity
One unit will oxidize 1.0 umole of glycerol to dihydroxyacetone per minute at pH 8.0 at 25 deg C. Specific activity: > 14 units/ml.
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Assay Information
(1) Prepare a 200 ul reaction mix with final concentrations of 93mM Glycine, 93mM Potassium chloride, 2375mM Glycerol, 3mM b-NAD. (2) Equilibrate to 25 deg C and monitor at A340nm until the reading is constant using a spectrophotometer. (3) Add 20 ul of recombinant GLDA protein at different concentrations (0.2 ug, 0.1 ug, 0.05 ug) in 180 ul of the prepared reaction buffer. (4) Invert to mix and read the decrease at A340nm for 10 minutes.
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